蛋白質高級結構ppt課件(編輯修改稿)
2025-02-13 18:57 本頁面
【文章內容簡介】 ount of work required, Perutz and Kendrew faced two major problems that had to be solved in order to obtain a structure: A. The phase determination (. what is up and down). This was achieved by the process of heavy atom (U or Pb) replacement. B. Obtaining adequate puter power for the massive number of putations, the Fourier transform calculations. The first electronic puters were not developed until after World War II. 復旦大學生物化學系 黃偉達 ? By 1957, Kendrew had measured 400 reflections, and from them, created a structure for myoglobin at the level of nm resolution. (No individual amino acid residues were visible) ? Later he analyzed 9600 reflections to produce a density map at nm resolution. ? Finally, in 1962 he analyzed 25,000 reflections to produce a density map at nm. Resolution of this level allowed assignment of 1200 of the 1260 nonhydrogen atoms. ? Today the technique of Xray crystallography has been refined such that preparing robust crystals of the appropriate size is the most difficult task in obtaining a molecular structure. The advent of arrays of detectors connected directly to powerful puters, permits the generation of molecular structures on the puter display in a matter of weeks. 復旦大學生物化學系 黃偉達 如何看立體圖 斗雞眼法 如何看立體圖 復旦大學生物化學系 黃偉達 蛋白質幾種不同的立體結構表示法 立體圖的不同表示方法 復旦大學生物化學系 黃偉達 XRay衍射決定蛋白質空間構象 X衍射決定蛋白質空間結構 復旦大學生物化學系 黃偉達 核磁共振是確定蛋白質分子在溶液中的動態(tài)結構的唯一方法 核磁共振研究蛋白質的立體結構 Kurt Wuthrich,瑞典科學家2022年獲得諾貝爾獎 復旦大學生物化學系 黃偉達 氫原子的核磁共振譜 復旦大學生物化學系 黃偉達 蛋白質的四級結構 蛋白質與蛋白質的相互作用，蛋白質與核酸的相互作用，比較簡單的體系有血紅蛋白、限制性內切酶等，復雜體系有核糖體、病毒、肌肉蛋白等。 復旦大學生物化學系 黃偉達 DNA和蛋白質的相互作用也是 四級結構 DNA和蛋白質的結合 復旦大學生物化學系 黃偉達 病毒是巨
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