《蛋白質(zhì)高級結(jié)構(gòu)》ppt課件-文庫吧
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【正文】 es. ? 1928: Kathleen Lonsdale reports the structure of benzene as having six equal sized bonds instead of alternating double and single bonds. ? 1935: J. M. Robertson et al. solve the structures of pthalocyanins, the first case of a plex anic molecule solved independently by crystallography. ? 1948: Bijvoet et al. solve strychnine, perhaps the first case in which crystallography decided between alternatives proposed by anic chemists. ? 1950: Bijvoet et al. establish the absloute configurations of dextro and laevo pounds with NaRb tartrate. ? 194957: Dorothy Crowfoot Hodgkin et al. solved the structures of penicillin (1949) and vitamin B12 (1957). She won the Nobel Prize in Chemistry in 1964. XRAY DIFFRACTION技術(shù)的歷史 復(fù)旦大學(xué)生物化學(xué)系 黃偉達(dá) XRAY DIFFRACTION 研究蛋白立體結(jié)構(gòu) 1936年 Max F. Perutz開始用 X光衍射技術(shù)來研究血紅蛋白的立體結(jié)構(gòu) 1945年 Perutz的學(xué)生 J. 1957年肌紅蛋白的結(jié)構(gòu)確定 , 1959年血紅蛋白結(jié)構(gòu)解明 兩人于 1962年獲得諾貝爾獎(jiǎng) . 復(fù)旦大學(xué)生物化學(xué)系 黃偉達(dá) Kendrew Perutz L. Braggs 復(fù)旦大學(xué)生物化學(xué)系 黃偉達(dá) Laboratory procedure ? Must have a purified protein (or nucleic acid) ? Must be able to crystallize this molecule into a crystal from that is stable to irradiation by Xrays ? Mount the crystal in the Xray machine ? Collect diffracted radiation on film or into an array of detectors ? Use a puter to carry out Fourier transforms on this density data ? From these spots (reflections) and the Fourier transform, a contour map of electron density is created ? An actual physical model is then constructed so as to place the nuclei of each of the nonhydrogen atoms in the center of one of each of the areas of high electron density (originally done by hand is now done by puter). ? In addition to the immense am
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