【正文】 斬首上胚軸；直接器官發(fā)生；植株再生；遺傳轉(zhuǎn)化新疆棉花高效再生體系建立及其遺傳轉(zhuǎn)化影響因素的研究IVABSTRACTCotton is an important economical crop all over the world. Requirement for cotton varities’ characters is plicated, because phases of its growth and development nicely was consistent with seasons of high frequency of various natural disaster. The conventional breeding proposal is faced with spareness of germ plasm resource and trouble of remote ancestors’crosses, which make good characters’ bination be greatly inconvenience, especially traits of high yield, good quality and mutiresistance to diseases and insects. So more and more scientific researchers pay attention to the study on improving cotton by geic engineering technology. But many obstacles still exist in development of cotton biology at present, for example somatic embryogenesis of cotton is geypedependent, tissue culture of cotton is low efficiency (take time and energy), geic transformation technology is intricate (repeatness and transformation are low), and variation of transgenic cotton’s agricultural traits is plex, and so on. These questions have bee hindrance and choke point of cotton biology, validatation of cotton function gene and cotton relative gene clone. As the problems narrated above, The paper deal with four different varieties of Xinjiang cotton, Study on high efficiency plant regeneration system and the influence factors geic transformation of Xinjiang Cotton Varieties in primary research results are as follows.1 The research deal with four different varieties of Xinjiang cotton, epicotyls act as the explants in explants were cultured on four different shoot inducement culture mediums. In the culture medium of MSB + 6BA + NAA But when the concentration of 6BA is added,the vitrification phenomenon is pricked up. 2 The research deal with four different varieties of Xinjiang cotton, epicotyls act as the explants in explants were cultured on four different shoot inducement culture the different shoot inducement culture mediums, In the culture medium of MSB + KT + NAA had the highest ratio of producing multiple the ratio of producing multiple shoots are all lower than the pounding of 6BA and growth instance of multiple shoots is disproportion. 3 Rooting was induced in 1/4MS added 10 mg/L IBA is the best than any other rooting culture mediums,and its ratio can reach % Shoot induction of cotton epicotyl is geypeindependent, the results were not appeared much difference between these cultivars. It is showed that multiple shoots induction was not under the control of different plant regeneration system is high efficiency, short cycle and maneuverable.4 The agrobacteriummediated gene transformation procedure was discussed in detail. The factors, including precultivation time, immersion time, cocultivation periods and concentration of acetosyringone (AS), The optimized transformation protocol was obtained when epicotyl explants precultured for 3~5 days on regeneration medium were immersed into the diluted Agrobacterium suspension containing 100mg/L AS for 15 min and then were cocultured for 2 days with Agrobacterium and were finally subcultured on Vselection medium after reinforcing cultivation for 3 days on the regeneration medium containing 800 mg/L cefotaxime and 50mg/L Kan.5 From treated epicotyl by agrobacteriummediated transformation,resistant plantlets have been obtained after kan resistance screening．Under irradiation of UV,the leaf of resistant plantlets were primary inspected,the green fluorescence could be observed from the leaf of resistant plantlets.Key words: Cotton(Gossypium hirsutum L. and L.)。結(jié)果表明：預(yù)培養(yǎng)3~5天的上胚軸在添加100mg/L乙酰丁香酮的農(nóng)桿菌重懸液中浸染15 min后共培養(yǎng)2天，經(jīng)過 3天的恢復(fù)培養(yǎng)之后用包含50 mg/L卡那霉素和800 mg/L頭孢霉素的篩選培養(yǎng)基進(jìn)行篩選是較為理想的遺傳轉(zhuǎn)化條件。以上胚軸為外植體，對棉花建立了操作比較簡單，系統(tǒng)變異小，遺傳穩(wěn)定性好，周期短，不依賴基因型的高效直接再生植株方法。2 對供試的四個材料的上胚軸進(jìn)行處理，采用KT和NAA的激素組合，進(jìn)行多芽誘導(dǎo)，以KT + NAA ， 但誘導(dǎo)出的多芽長勢不均，且多芽誘導(dǎo)率低于6BA和NAA的激素組合。取得的主要研究結(jié)果如下：1 對供試的四個材料的上胚軸進(jìn)行處理，采用6BA和NAA的激素組合，進(jìn)行多芽誘導(dǎo)，隨著6BA濃度的提高，有部分玻璃化苗的出現(xiàn)。然而目前棉花生物技術(shù)的發(fā)展仍然存在著很多障礙，如棉花體細(xì)胞胚胎發(fā)生的基因型依賴性、棉花組織培養(yǎng)的低效性（耗時、耗工） 、基因轉(zhuǎn)化技術(shù)的復(fù)雜性（難重復(fù)，轉(zhuǎn)化率低）和轉(zhuǎn)基因棉花農(nóng)藝性狀的變異等等，這些問題已經(jīng)成為棉花生物技術(shù)、棉花功能基因的驗證和棉花相關(guān)基因克隆等研究領(lǐng)域的障礙和瓶頸。作者簽名： 日期： 年 月 日導(dǎo)師簽名： 日期： 年 月 日指導(dǎo)教師評閱書指導(dǎo)教師評價：一、撰寫（設(shè)計）過程學(xué)生在論文（設(shè)計）過程中的治學(xué)態(tài)度、工作精神□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格學(xué)生掌握專業(yè)知識、技能的扎實程度□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格學(xué)生綜合運用所學(xué)知識和專業(yè)技能分析和解決問題的能力□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格研究方法的科學(xué)性；技術(shù)線路的可行性；設(shè)計方案的合理性□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格完成畢業(yè)論文（設(shè)計）期間的出勤情況□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格二、論文（設(shè)計）質(zhì)量論文（設(shè)計）的整體結(jié)構(gòu)是否符合撰寫規(guī)范？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格是否完成指定的論文（設(shè)計）任務(wù)（包括裝訂及附件）？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格三、論文（設(shè)計）水平論文（設(shè)計）的理論意義或?qū)鉀Q實際問題的指導(dǎo)意義□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格論文的觀念是否有新意？設(shè)計是否有創(chuàng)意？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格論文（設(shè)計說明書）所體現(xiàn)的整體水平□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格建議成績：□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格（在所選等級前的□內(nèi)畫“√”）指導(dǎo)教師： （簽名） 單位： （蓋章）年 月 日評閱教師評閱書評閱教師評價：一、論文（設(shè)計）質(zhì)量論文（設(shè)計）的整體結(jié)構(gòu)是否符合撰寫規(guī)范？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格是否完成指定的論文（設(shè)計）任務(wù)（包括裝訂及附件）？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格二、論文（設(shè)計）水平論文（設(shè)計）的理論意義或?qū)鉀Q實際問題的指導(dǎo)意義□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格論文的觀念是否有新意？設(shè)計是否有創(chuàng)意？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格論文（設(shè)計說明書）所體現(xiàn)的整體水平□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格建議成績：□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格（在所選等級前的□內(nèi)畫“√”）評閱教師： （簽名） 單位： （蓋章）年 月 日教研室（或答辯小組）及教學(xué)系意見新疆棉花高效再生體系建立及其遺傳轉(zhuǎn)化影響因素的研究II教研室（或答辯小組）評價：一、答辯過程畢業(yè)論文（設(shè)計）的基本要點和見解的敘述情況□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格對答辯問題的反應(yīng)、理解、表達(dá)情況□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格學(xué)生答辯過程中的精神狀態(tài)□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格二、論文（設(shè)計）質(zhì)量論文（設(shè)計）的整體結(jié)構(gòu)是否符合撰寫規(guī)范？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格是否完成指定的論文（設(shè)計）任務(wù)（包括裝訂及附件）？□ 優(yōu) □ 良 □ 中 □ 及格 □ 不及格三、論文（設(shè)計）水平論文（設(shè)計）的理論意義或?qū)鉀Q實際問題的指導(dǎo)意義□ 優(yōu) □ 良