【正文】 D果聚糖酶，又叫 β果聚糖酶， β2,1D果聚糖水解酶，可以在一定的溫度條件下水解菊粉成果糖或低聚果糖 [10]。進一步篩選性能優(yōu)良的菊粉酶高產(chǎn)菌仍舊十分重要。目前均停留于實驗室階段，限制因子在于：產(chǎn)酶菌株菊粉酶產(chǎn)量低，產(chǎn)酶成本高。Screening the main factor that affect inulinase activity in the fermentation process by PlackettBurmen test from soybean pulp addition,inulin addition,(NH4)2SO4 addition,K2HPO4 addition,Twain addition,MgCl addition,initial pH,CaCl2 addition,and the main factors are soybean pulp addition,initial pH and CaCl2 addition,Design response surface methodology explore the optimal fermentation conditions as follows:corn syrup %,inulin 2%,(NH4)2SO4 %,dipotassium hydrogen phosphate %,tween80 %,magnesium chloride %, the initial pH value of ,calcium chloride %。本研究從低溫保存的腐爛菊芋上分離出耐低溫菊粉酶產(chǎn)生菌，以 DNS 比色法從 33株菌株中篩選出菊粉酶高產(chǎn)菌株即 2 號菌株，其酶活力為 ；通過 PlackettBurmen 試驗從豆粕添加量，菊粉添加量，(NH 4) 2SO4 添加量，磷酸氫二鉀添加量，吐溫添加量，氯化鎂添加量，初始 pH 值，氯化鈣添加量中篩選出影響發(fā)酵過程中菊粉酶活力的主要因素，即為豆粕添加量、初始 pH 值及氯化鈣添加量，再通過設計響應面試驗探究其最優(yōu)發(fā)酵條件為：豆粕添加量 %，菊粉添加量 2%，(NH 4)2SO4 %，磷酸氫二鉀%，吐溫 %，氯化鎂 %，初始 pH 值 ，氯化鈣添加量 %；得到的此最佳發(fā)酵條件下的酶活力為 。：耐低溫菊粉酶產(chǎn)生菌的分離及發(fā)酵條件優(yōu)化研究外文題目：ISOLATION OF LOWTEMPERATURERESISTANT INULINASE PRODUCING STRAIN AND OPTIMIZATION OF FERMENTATION CONDITIONS 畢業(yè)設計（論文）共 50 頁（其中：外文文獻及譯文 23 頁）圖紙共 0 張 完成日期 2022 年６月 答辯日期 2022 年 6 月摘要在如今物質豐富，人們注重營養(yǎng)保健的年代，菊粉酶作為一種能將菊粉降解為高純度果糖或低聚果糖的酶制劑，其工業(yè)化大規(guī)模生產(chǎn)越來越受到科研界及商業(yè)界的重視。目前國外對微生物菊粉酶進行了許多研究，包括菌種的篩選、產(chǎn)酶條件及酶的性質的研究等，我國關于菊粉酶的研究目前還停滯在實驗室階段，由于酶的產(chǎn)量低、生產(chǎn)成本高還未進行工業(yè)化應用,因此進一步篩選性能優(yōu)良的高產(chǎn)酶菌株仍然十分重要。關鍵詞：菊粉酶；高產(chǎn)菌株；發(fā)酵條件優(yōu)化AbstractIn the era of material abundance,people pay more attention to nutrition and health,inulinase as a Enzyme preparation which can degradate inulin to high purity fructose or lowmolecularweight fructos,it largescale production attract more and more attention by the research munity and the business present foreign study so much on microbial inulinase , including strains isolation, fermentation conditions and the nature of the enzyme, our studies on inulinase is still stuck in the laboratory stage, due to the low yield of enzyme and high production costs,it not yet industrialized application. Therefore, further screening of excellent performance, highyield enzyme strains are still very important.The study separation lowtemperatureresistant inulinase producing strains from the decayed artichoke kept at low temperature ,screening high yield inulin enzyme strains from the 33 strains by the method of DNS colorimetric,and the result show that 2nd strain enzyme is the highest yield Inulin enzyme ,enzyme activity lead to 。it enzyme activity is Key words:inulin enzyme；h ighyielding strains；optimal fermentation conditions目錄1 引言 ........................................................................................................................1 菊粉酶概述 .........................................................................................................3 研究現(xiàn)狀 .............................................................................................................6 研究的目的意義 ................................................................................................62 材料與方法 ............................................................................................................7 樣品 ....................................................................................................................7 培養(yǎng)基 ................................................................................................................7 主要試劑 ......................................................7 主要儀 器設備 .....................................................................................................8 技術路線及操作要點 .........................................................................................8 技術路線 ..........................................................................................................8 操作要點 ..........................................................................................................9 研究內容 ...........................................................................................................10 菌種分離 ........................................................................................................10 菌種篩選 ........................................................................................................11 發(fā)酵條件優(yōu)化研究 ........................................................................................11 菊粉酶活力的測定方法 ..................................................................................123 試驗結果與 分析 .........................................................