基因工程課程簡(jiǎn)介-文庫(kù)吧資料
2024-11-13 19:12本頁(yè)面
【正文】 teins Native and fusion proteins Yeast expression systems The baculovirus expression system Mammalian cell lines Protein engineering Examples of biotechnological applications of rDNA technology Production of enzymes The BST story Therapeutic products for use in human healthcare 本章要點(diǎn)：掌握基因工程生產(chǎn)蛋白原理和方法。 （重點(diǎn)） Part III Geic Engineering in Action (12hrs) Chapter 9 Understanding genes and genomes (self study) Analysis of gene structure and function A closer look at sequences Finding important regions of genes Investigation gene expression From genes to genomes Analysing genomes Mapping genomes Genome sequencing Sequencing technology Genome projects The human genome project Whose genome, and how many genes does it contain Geic and physical maps of the human genome Deriving and assembling the sequence What next? 本章要點(diǎn)：掌握基因結(jié)構(gòu)與功能。（重點(diǎn)） Chapter 8 Selection, Screening and analysis of rebinants (6hrs) Geic selection and screening methods The use of chromogenic substrates Insertional inactivation Complementation of defined mutations Other geic selection methods Screening using nucleic acid hybridisation Nucleic acid and probes Screening clone banks Immunological screening for expressed genes Analysis of cloned genes Characterisation based on mRNA translation in vitro Restriction mappin Blotting techniques DNA sequencing 本章要點(diǎn)：掌握常用的抗菌素抗性篩選、酶切篩選、 雜交篩選、 酶聯(lián)免疫吸附測(cè)定（ ELISA）、免疫印跡（ western blotting）法的原理。 了解酵母人 工染色體和細(xì)菌人工染色體的結(jié)構(gòu)和工作原理。 掌握基因轉(zhuǎn)入受體細(xì)胞的基本方法和原理。 掌握載體的基本結(jié)構(gòu)和質(zhì)粒載體、噬菌體載體的特點(diǎn)、構(gòu)建原理。了解 DNA連接酶和修飾酶在基因操作中的用途。 （次重點(diǎn)） Chapter 4 The tools in the geic engineering (3hrs) Restriction enzyme— cutting DNA Type II restriction endonucleases Use of restriction endonucleases Restriction mapping DNA modifying enzymes Nucleases Polymerases Enzymes that modify the ends of DNA molecules DNA ligase— joining DNA molecules 本章要點(diǎn)：掌握限 II 型制性核酸內(nèi)切酶的切割原理和部分常用的限制性核酸內(nèi)
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