《基因工程》課程簡(jiǎn)介-文庫(kù)吧
2024-10-16 19:12 本頁(yè)面
【正文】 of the geic engineering The course content and how to learn the course? 本章要點(diǎn)：掌握基因工程的含義和基因工程誕生的理論基礎(chǔ)與技術(shù)突破。了解基因工程的發(fā)展和在社會(huì)生產(chǎn)中的應(yīng)用。 （次重點(diǎn)） Part I: The basis of geic engingeering Chapter 2 The molecular biology basis of geic engineering (3hrs) The structure of DNA and RNA The Central Dogma of molecular biology Gene and Gene structure Gene structure in prokaryotes Gene structure in eukaryotes Gene expression Genomes Genome size and plexity Genome anization 本章要點(diǎn)：掌握 DNA、 RNA 結(jié)構(gòu)、分子生物學(xué)中 心法則、原核生物和真核生物基因結(jié)構(gòu)和基因組基本組成。 Chapter 3 Working with nucleic acids (3hrs) Isolation of DNA and RNA Handling and quantification of nucleic acids Radiolabelling of nucleic acids End labeling Nick translation Labelling by primer extension Nucleic acid hybridisation Gel electrophoresis DNA sequencing Maxam— Gilbert (chemical) sequencing Sanger— Coulson (dideoxy or enzymatic) sequencing Electrophoresis and reading of sequences 本章要點(diǎn)：掌握 DNA 和 RNA提取、 DNA電泳、核酸放射性標(biāo)記、分子雜交和 DNA序列測(cè)定的技術(shù)原理。 （次重點(diǎn)） Chapter 4 The tools in the geic engineering (3hrs) Restriction enzyme— cutting DNA Type II restriction endonucleases Use of restriction endonucleases Restriction mapping DNA modifying enzymes Nucleases Polymerases Enzymes that modify the ends of DNA molecules DNA ligase— joining DNA molecules 本章要點(diǎn)：掌握限 II 型制性核酸內(nèi)切酶的切割原理和部分常用的限制性核酸內(nèi)切酶的識(shí)別位點(diǎn)與切割末端。掌握常用的 DNA聚合酶的特性和用途。了解 DNA連接酶和修飾酶在基因操作中的用途。 （重點(diǎn)） Part II: The methodology of gene manipulation Chapter 5 Host cells and vectors (8hrs) Host cells types Prokaryotic hosts Eukaryotic hotst Plasmid vectors for use in What are plasmids? Basic cloning plasmids Slightly more exotic plasmid vectors Bacteriophage vectors for use in What are bacteriophages? Vectors based on bacteriophage λ Vectors based on bacteriophage M13 Other vectors Hybrid plasmid/phage vectors Vectors for use in eukaryotic cells Artificial chromosomes Getting DNA into cells
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