【正文】
. Bioprocess and Biosystems Engineering,1998,18(6):457461.[21] Yan B, Xu A, Zhang W, et al. Accumulation of 2Keto1Gulonate at 33 ℃ by a Thermotolerant Gluconobacter Oxydans Mutant Obtained by Ion Beam Implantation. Plasma Science amp。 Technology,2006,8(2):237241.[22] Moonmangmee D, Adachi O, Ano Y, et al. Isolation and Characterization of Thermotolerant Gluconobacter Strains Catalyzing Oxidative Fermentation at Higher Temperatures. Biosci Biotechnol Biochem,2000,64(11):230615. [23] Yang FC, Lim YH. Kinetic Study of the Bioconversion of DSorbitol to LSorbose by Acetobacter Pasteurianus. Process Biochemistry,1997,32(3):233236.[24] Yang F, Jia Q, Xiong ZH, et al. Complete Genome Analysis of Ketogulonigenium Sp. Wb0104. Chinese Science Bulletin,2006,51(8):941945.[25] 陳芳, 陳策實(shí), 李越, 尹光琳. 歐文氏菌2酮基醛糖還原酶基因敲除的研究. 微生物學(xué)報(bào), 2000, 40(5): 475481.Chen F, Chen CS, Li Y, Yin GL. Studyies on gene knocking out of 2keto aldose reductases from Erwinia sp. SCB125. Acta Microbiologica Sinica, 2000, 40(5): 475481.[26] Sugisawa T, Hoshino T, Nomura S, et al. Isolation and Characterization of MembraneBound LSorbose Dehydrogenase from GluconobacterMelanogenus Uv10. Agricultural and Biological Chemistry,1991,55(2):363370.[27] 王虹. 維生素C兩步發(fā)酵中關(guān)鍵步驟的分子生物學(xué)研究. 軍事醫(yī)學(xué)科學(xué)院生物工程研究所碩士論文, 2005, 5.Wang H. Study on the molecular biology of the key step in twostep fermentation process of vitamin C. Master degree thesis of Institute of Biotechnology , Academy of Military Medical Sciences, 2005, 5.[28] Anderson S, Marks CB, Lazarus R, et al. Production of 2KetoLGulonate, an Intermediate in LAscorbate Synthesis, by a Genetically Modffied Erwinia Herbicola. Science,1985,230(4722):144149.[29] 蔣雨楊, 郭振勇, 張成剛. 2酮L古龍酸還原酶分離純化及其理化、酶學(xué)性質(zhì)的研究. 生物工程學(xué)報(bào), 1997, 13(4): 400405.Jiang YY, Guo ZY, Zhang CG. Study on the purification of 2ketoLgulonate reductase and its physical 、chemical and enzymic properties. Chinese Journal of Biotechnology, 1997, 13(4): 400405.[30] Banta S, Anderson S. Verification of a Novel NadhBinding Motif: Combinatorial Mutagenesis of Three Amino Acids in the CofactorBinding Pocket of Corynebacterium 2,5DiketoDGluconic Acid Reductase. J Mol Evol,2002,55(6):62331.[31] Banta S, Swanson BA, Wu S, et al. Optimizing an Artificial Metabolic Pathway: Engineering the Cofactor Specificity of Corynebacterium 2,5DiketoDGluconic Acid Reductase for Use in Vitamin C Biosynthesis. Biochemistry,2002,41(20):62266236.[32] 馮樹(shù), 張舟, 張成剛, 張忠澤. 混合培養(yǎng)中巨大芽孢桿菌對(duì)氧化葡萄糖酸桿菌的作用. 應(yīng)用生態(tài)學(xué)報(bào), 2000, 11(1): 119122.Feng S, Zhang Z, Zhang CG, Zhang ZZ. Effect of Bacillus megaterium on Gluconobacter oxydans in mixed culture. Chinese Journal of Applied Ecology, 2000, 11(1): 119122.[33]呂淑霞, 馮樹(shù), 張忠澤, 劉陽(yáng), 謝占武, 安海英. Vc二步發(fā)酵中伴生菌的作用機(jī)制. 微生物學(xué)通報(bào), 2001, 28(5): 1013.Lu SX, Feng S, Zhang ZZ, Liu Y, Xie ZW, An HY. The effect of Bacillus megaterium in vitamin C twostep fermentation. Microbiology, 2001, 28(5): 1013.[34] Saito Y, Ishii Y, Hayashi H, et al. Direct Fermentation of 2KetoLGulonic Acid in Rebinant Gluconobacter Oxydans. Biotechnology and Bioengineering,1998,58(23):309315.[35] 林紅雨, 陳策實(shí), 尹光琳. 歐文氏菌和棒桿菌的屬間融合研究. 微生物學(xué)通報(bào), 1999,26(1):36.Lin HY, Chen CS, Yin GL. Intergeneric Cell Fusion of Corynebacterium and Erwinia. Microbiology,1999,26(1):,也不能閑死在家里!寧可去碰壁,也不能面壁。是狼就要練好牙,是羊就要練好腿。什么是奮斗?奮斗就是每天很難,可一年一年卻越來(lái)越容易。不奮斗就是每天都很容易,可一年一年越來(lái)越難。能干的人,不在情緒上計(jì)較,只在做事上認(rèn)真;無(wú)能的人!不在做事上認(rèn)真,只在情緒上計(jì)較。拼一個(gè)春夏秋冬!贏一個(gè)無(wú)悔人生!早安!—————獻(xiàn)給所有努力的人. 學(xué)習(xí)好幫手