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【正文】 onstiutively expresses SEAP in most cell types, which makes it ieal for estabilshing transfection efficiency and optimizing your SEAP assay detection method procedureAfter transfection: it is important to remove serum from the meium to ensure properinduction of th reporter Serum induces various signaling pathways causing high background.About stimulus: perform a time course study by collecting samples at various time points.SEAPcan collect samples from a single cell culture Luciferasemust set up multiple cell cultures to
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