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年(北京醫(yī)科大學(xué)人民醫(yī)院婦科腫瘤中心,北京 100044)參考文獻(xiàn)[1]錢和年,馮捷,[J].北京醫(yī)科大學(xué)學(xué)報,1987,19(4):211~213.[2]Qian HN, Lu WY. Antiidiotypic monoclonal antibodies against antiovarian carcinoma monoclonal antibody COC1669: Generation and application[J]. CMJ,1994,107(2):99103.[3]呂文英,錢和年,[J].中華婦產(chǎn)科雜志,1994,29(1):27~29.[4]Qian HN, Wang JL, Feng J, et al. Vaccination with monoclonal antiidiotypic antibody on immunopetent mice bearing human ovarion cancer[J]. CMJ,1997,110(4):259263.[5]張香云,錢和年,邱并生,[J].北京醫(yī)科大學(xué)學(xué)報,1996,28(4):252~255.[6]Chatterjee BM, Mukerjee S, Biddle W, et al. Murine monoclonal antiidiotype antibody as a potential network antigen for human carcinoembryonic antigen[J]. J Immunol, 1990,145(8):27582765.[7]Tao MH, Levy R. Idiotype/granulocytemacrophage clonystimulating factor fusion protein as a vaccine for Bcell lymphoma[J]. Nature,1993,362(6422):755758.[8]楊飛雪,錢和年,/hGMCSF融合蛋白對體外免疫細(xì)胞的刺激作用[J].北京醫(yī)科大學(xué)學(xué)報,1999;31(1):9~12.[9]Gough N M, Metcalf D, Gough J, et al. Structure and expression of the mRNA for murine granulocytemacrophage colony stimulating factor[J]. EMBO J, 1985,4(3):645653.[10]Jerne NK. Towards a network theory of the immune system[J]. Ann Immunol(Paris),1974,125C(12):373389.[11]Bona CA, HeberKatz E, Paul W E. Idiotypeantiidiotype regulation immunization with a levanbinding myeloma protein leads to the appearance of autoanti(antiidiotype) antibodies and to the activation of silent clones[J]. J Exp Med, 1981,153(4):951967.[12]Wagner U, Schlebusch H, Kohler, et al. Immunological responses to the tumorassociated antigen CA125 in patients with advanced ovarian cancer induced by the murine monoclonal antiidiotypic vaccine ACA125[J]. Hybridoma,1997,16(1):3340.[13]Fargerberg J, Steinitz M, Wigzell H, et al. Human antiidiotypic antibodies induced a humoral and cellular immune response against a colorectal carcinomaassociated antigen in patients[J]. Proc Natl Acad Sci USA,1995,92(11):47734777.【收稿日期】19990625 【修稿日期】19990910內(nèi)容總結(jié) (1)卵巢癌抗獨特型單鏈抗體6B11ScFv/mGMCSF融合蛋白的構(gòu)建、表達(dá)及活性測定