多色流式細胞(facs)調(diào)補償與圈門-資料下載頁
2025-08-05 04:40本頁面
【正文】 ,000 lymphocytes collected Number of Events vs Measurement Precision CD4+ T cells CD8+ T cells 14 events = % 73 events = % 8 events = % 51 events = % Compensation, Controls, and Data Collection 231353600 Rev. 01 19 Statistical Significance of Results Determining the Number of Events to Collect Number of Relevant Events to Collect % Background (False +) Lowest % Positive 90% power, p 99% power, p 260,000 720,000 32,000 90,000 12,000 32,000 67,000 190,000 16,000 45,000 170,000 480,000 23,000 63,000 33,000 93,000 52,000 140,000 86,000 240,000 160,000 450,000 17,000 46,000 26,000 72,000 Compensation, Controls, and Data Collection 231353600 Rev. 01 20 Data Collection Discussion ? Storage Gates and Stopping Gates ? Global Worksheets vs Normal Worksheets ? Templates Compensation, Controls, and Data Collection 231353600 Rev. 01 21 Experiment Setup and Record Sample Data Exercise (see workbook for additional detail) 1. Create an experiment. 2. Apply application settings. 3. Create pensation controls, calculate and QC pensation. 4. Collect sample data. ? Negative control ? Activated sample
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