大腸桿菌耐藥株的體外誘導(dǎo)及其acra和mara基因分析-資料下載頁
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【正文】 rug transporters in microrganisms. Biochem Biophs Acta,1998,13:1. [8]Nikaido H. Antibioti cresistance caused by gramnegtive multidrug efflux pumps. Cli Efect Dis,1998,27(suppl. 1):S32. [9]Paulsen I T,Skurray R A, Tametal R. The SMR family:a novel family of multidrug efflux protein in volved with the efflux of lipophilic drugs. Mol Microbiol,1999,19:1175. [10]Notka F,Linde H J,Dankesreiter A, et al. AC terminal1 8 amino acid deletion in MarR in a clinical isolate of Escherichia coli reduces MarR binding properties and in creases the MIC of ciprofloxacin.. J Antimicrob Chemother, 2002,49(1):41.[11]Kaatz G W,Seo S M,Ruble C A. Effluxmediated fluoroquinolone resistance in Staphylococcus aureus. Antimicrob Agents Chemother,1993, 37:1086. [12]Hachler H, Cohen S P,amp。 Levy S a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli. Joural of Bacteriology,1991,173:5532. [13]張永信. 遠東地區(qū)細菌耐藥狀況與對策. 中國抗菌藥雜志. 1994,19(5):391. [14]倪語星,洪秀華. 細菌耐藥性監(jiān)測與抗感染治療,人民軍醫(yī)出版社,. [15]Asako H,Nakajima H,Kobayashi K,et al. Organic solvent tolerance and antibiotic resistance increased by over expression of marA in Escherichia coli. Appl Environ Microbiol, 1997,63(4):1428. 圖1 SEMR、SEICI、ATCC25922菌株基因組DNA提取物的瓊脂糖電泳Figure 1 gelose gel electrophoresis with Genome DNA of ATCC25922,SEMR and SEICI.1:λ－Hind Ⅲ digest DNA Marker。 2: ATCC25922。 3: SEMR。 4: SEICI 圖4 克隆質(zhì)粒的PCR鑒定Figure 4 The identification of cloning plasmid monitoring with PCR1,2,3:marA of ATCC25922,SEMR,SEICI。M:DL2,000 DNA Marker。 4,5,6:acrA of ATCC25922,SEMR,SEICI.圖2 acrA 基因的PCR擴增結(jié)果Figure 2 The amplification of acrA gene with PCR 1:ATCC25922。 2:SEMR。 3:SEICI。 M:DL2,000 DNA Marker 1: acrA。 2 :DL2,000 DNA Marker圖3 marA 基因的PCR擴增結(jié)果Figure 3 The amplification of acrA gene with PCR 1:ATCC25922。 2:SEMR。 3:SEICI。M:DL2,000 DNA Marker圖5 克隆質(zhì)粒的酶切鑒定Figure 5 The identification of Cloning plasmid monitoring with restriction enzyme digestion1,2,3:acrA－pGEMT of TCC25922,SEMR,SEICI 4:λ－Hind Ⅲ digest DNA Marker。 圖6 克隆質(zhì)粒的酶切鑒定Figure 6 The identification of Cloning plasmid monitoring with restriction enzyme digestion 1:λ－Hind Ⅲ Marker。 2,3,4: marA－pGEMTof ATCC25922,SEMR,SEICI。5: DL2,000DNA Marker7
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