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蛋白質生化分離技術-資料下載頁

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【正文】 ing bioseparation (11) ? C Protein refolding and inclusion bodies (9) ? Quality control ? (1) pressure from regulatory agencies ? (2) public awareness ? (3) expanded and fierce petition ? (4) safety factors ? (5) specific action and minimal side effect requirements V Some factors influencing bioseparation (12) ? C Protein refolding and inclusion bodies (10) ? Protein stability in finished formulation ? (1) surfactants or stabilizers(sugars,amino acids and fatty acid) ? (2) pH ? (3) antioxidants (containing sulfur amino acids) ? (4) cryoprotectants (minimizing freezing loss) ? (5) lyoprotectants (minimizing freezydrying loss) New words and vocabulary (1) ? Synchronized (同步的) ? Variant(變量) ? Conformation (構象) ? Variance (變異) ? Feedstocks(給料) ? Construction (構建) ? Polypeptide ( 多肽) ? Hypothetical (假定) ? native (未觸動的,天然的) ? Mitochondrial (線料體) ? Malate (蘋果酸鹽) New words and vocabulary (2) ? Dehydrogenase (脫氫酶) ? In vitro (體外) ? Refractile (能折射的) ? Refolded (再折疊) ? Underscored (在 … 下劃線) ? Intentionally (故意地) ? Review (評論,審閱) ? Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDSPAGE) ? Rodlike ? DNA topoisomerase ( DNA topoisomerase) ? Estrogen receptor (雌激素受體 ) ? Fructosyltransferase (果糖基轉移酶) New words and vocabulary (3) ? Supersaturation (超飽和) ? Nicking (基因巧合,切割) ? Deamidation (脫酰胺) ? Alleviate (緩和,減輕) ? Tankage (桶槽容量) ? Behooves (適宜,應該) ? Erythropoietin (促紅細胞生成素) ? Pitfalls (缺陷) ? abound (大量存在的) ? Segregation (隔離) New words and vocabulary (4) ? Premium (額外費用) ? Filling ( 填補) ? Be bound to (一定要) ? Fierce (殘酷的) ? Reluctance (勉強) ? Parenteral (注射用的,非腸道的) ? Prone to (傾向于) ? Colony stimulating factor (集落刺激因子) Question ? 1 Why did some bioseparation experiments were carried out under microgravity or zero gravity in space ? ? 2 Why intentionally denature a protein in some cases during bioseparation? ? 3 How stabilize proteins in finished formulation? ? 4 What advantages process integration has? ? 5 concept: inclusion, renaturation, inactivation, integration, fuse protein, aggregation?
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