醫(yī)藥衛(wèi)生]吸入麻醉藥肝保護(hù)作用-資料下載頁(yè)
2025-01-04 08:07本頁(yè)面
【正文】 Lung pathology was attenuated to a great extent. Original magnification: 400. ／干重比（ wet/dry weight radio） Figure 2. Lung tissue wet/dry weight ratio. *p vs. sham group。 p vs. sham group。 △ p vs. I/R+S group or I/R+V group. ** △0123456Sham I/R+S I/R+V I/R+Elung wet/dry weight radioFigure 4. RTPCR analysis of ICAM1 mRNA expression in lung after hepatic I/R. *p vs. sham group。 △ p vs. I/R+S group or I/R+V group. GAPDHH ICAM1 3, 肺組織 ICAM1mRNA表達(dá) 的 RTPCR半定量檢測(cè) * △**01sham I/R+S I/R+V I/R+ERelative band intensityFig Effects of emulsified isoflurane pretreatment on the Tnfαlevels in lung tissue and plasma after hepatic ischemiareperfusion in rats , *p vs. sham group。 △ p vs. I/R+S group or I/R+V group. ５ . 血漿和肺組織中促炎細(xì)胞因子 TNFα 水平的變化： ***△020406080100120140160sham I/R I/R+V I/R+Eplasma TNFα(pg/ml)* △** 0100200300400500600sham I/R I/R+V I/R+Elung TNFα(pg/ml)5． 肺組織 NFκ B P65的免疫組織化學(xué)染色分析 Figure 5. Immunohistochemical detection of Lung NFκB p65. A, sham group: Weak NFκB p65 expression was observed. B, IR+S group: NFκB p65 expression was markedly increased. C, IR+V group: similary to IR+S group D, IR+E group. NFκB p65 expression was attenuated to a great extent pare to IR+S group and. IR+V group .Original magnification: 400. Sham I/R+S I/R+V I/R+E Fig 7. Effects of emulsified isoflurane pretreatment on NFκB p65 translocation in lung of after hepatic ischemiareperfusion. The top panel shows Western blot analysis for NFkB p65 in nuclear protein extracts from rat lung. The bottom panel shows relative densitometric units. Data are expressed as mean 177。 SD. In each experiment, the relative densitometric signal from the shamvehicle group was arbitrarily set to 1 *p vs. sham group。 △ p vs. I/R+S group or I/R+V group. NFκ B P65的免疫印跡分析 ***sham I/R+S I/R+V I/R+EBand density?肝臟缺血再灌注可介導(dǎo)遠(yuǎn)隔肺損傷 ?乳化異氟醚預(yù)處理可明顯減輕肝臟缺血再灌注介導(dǎo)的急性肺損傷及炎癥反應(yīng) ， 其機(jī)制可能與其抑制細(xì)胞間黏附分子 ICAM1的表達(dá) ， 減少中性粒細(xì)胞在肺組織的浸潤(rùn) ,抑制炎癥轉(zhuǎn)錄因子 NFκB 的活性有關(guān) 主要結(jié)論 小結(jié)和展望 異氟醚等為代表的吸入麻醉藥的肝臟保護(hù)作用是肯定的 ， 但發(fā)揮保護(hù)作用的最佳用藥時(shí)機(jī)及方式 ,最佳劑量 , 預(yù)處理的保護(hù)效應(yīng)窗 ， 后處理的作用和效應(yīng)窗 ， 相關(guān)作用靶位之間的聯(lián)系和相互作用 ,相關(guān)的信號(hào)轉(zhuǎn)導(dǎo)機(jī)制 ,同其他肝臟保護(hù)藥物或措施的相互作用 ，等都有待深入研究 。
教學(xué)課件相關(guān)推薦
鄂ICP備17016276號(hào)-1