【文章內(nèi)容簡(jiǎn)介】 是CE的檢測(cè)器靈敏度有些欠缺, 對(duì)常用的UV檢測(cè)器, 由于各砷化物的吸收系數(shù)小, 其檢測(cè)限僅為mg/ L水平, 這對(duì)質(zhì)量濃度為μg/ L 級(jí)的實(shí)際血樣、尿樣和環(huán)境樣品來(lái)說(shuō)是遠(yuǎn)不夠的,因此在一定程度上限制了其在形態(tài)分析中的應(yīng)用。但是近幾年人們通過(guò)衍生化, 或使CE 與HG ICP MS、HG ICP AES 或與激光誘導(dǎo)熒光檢測(cè)器(LIF) 聯(lián)用, 使CE在形態(tài)分析中的靈敏度得以提高[6 ] 。4 　結(jié)　語(yǔ)綜上所述, HPLC 和ICP MS、ICP AES 聯(lián)用是當(dāng)前對(duì)實(shí)際樣品分析中最常用的形態(tài)分析方法。ICP MS 的靈敏度較高, 已成為形態(tài)分析中最有力的一種分析工具, 但由于其售價(jià)比較昂貴, 還不能在一般分析實(shí)驗(yàn)室普及使用。氫化物發(fā)生原子光譜分析法靈敏度雖然較高, 但常常只能對(duì)部分砷化物進(jìn)行分析, 且前處理步驟比較復(fù)雜。CE 與ICP MS 的聯(lián)用是一種新技術(shù), 雖然CE的樣品用量和試劑用量都很少, 但I(xiàn)CP MS的價(jià)格和霧化器設(shè)計(jì)等原因決定著它的使用。激光誘導(dǎo)熒光(LIF)檢測(cè)器是一種十分靈敏的檢測(cè)裝置, 和ICP MS相比, 其價(jià)格低許多, 如果再輔以固相萃取等濃縮技術(shù), 則可達(dá)到需要的靈敏度。形態(tài)分析的研究趨勢(shì)就是利用高效能的分離技術(shù)達(dá)到各形態(tài)的有效分離, 并利用選擇性極強(qiáng)、靈敏度極高的元素特征檢測(cè)器達(dá)到超痕量形態(tài)的測(cè)定。參考文獻(xiàn):[1 ] Beard H C , Lyerly L A. Separation of arsenic from antimony and bismuth by solvent extraction [J ] . Anal Chem , 1991 ,33 (12) : 1781～1782.[2] Zhang X, Vanderbiesen V , Cubber A D. Accumeulation of arsenic species in serum of patients with chronic renaldisease[J ] . Clin Chem , 1996 , 42 (8) : 1231～1237.[3 ] 劉演兵, 韓恒斌. 砷形態(tài)分析方法研究進(jìn)展[J ] . 環(huán)境科學(xué)進(jìn)展, 1994 , 2 (4) : 1.[4] 馮樹(shù)屏. 砷的化學(xué)分析[M] . 北京: 中國(guó)環(huán)境科學(xué)出版社, 1992. 1.[5 ] 陳靜, 周黎明, 曲剛蓮. HPLC 聯(lián)用技術(shù)在環(huán)境砷形態(tài)分析上的應(yīng)用[J ] . 環(huán)境科學(xué)與技術(shù), 2003 , 26 (2) :60～62.[6 ] 張普敦, 許過(guò)旺, 魏復(fù)盛. 砷形態(tài)分析方法進(jìn)展[J ] . 分析化學(xué), 2001 , 29 (8) : 71～977.[7 ] 謝連宏, 劉樹(shù)森. 微波消解2氫化物發(fā)生原子吸收法測(cè)定食物中的砷[J ] . 中國(guó)衛(wèi)生檢驗(yàn)雜志, 1999 , 9(6) : 428～430.[8 ] 陳世忠. 氫化物發(fā)生電感耦合等離子體原子發(fā)射光譜法測(cè)定黃姜中的砷、銻和鉍[J ] . 微量元素與健康研究, 2003 , 20 (3) : 34～36.[9 ] 吳明嘉, 任紅星. 用毛細(xì)管電泳研究不同形態(tài)的砷[J ] . 分析化學(xué), 1996 , 24 (8) : 910～913.外文翻譯一原文Speciation analysis of inorganic arsenic by microchip capillaryelectrophoresis coupled with hydride generation atomicfluorescence spectrometry Feng Li a, DongDong Wang a, XiuPing Yan a,?, RongGuo Sub, JinMing Lin b,? Speciation analysis of inorganic arsenic by microchip capillary electrophoresis coupled with hydride generation atomicfluorescence spectrometry Journal of Chromatography A, 1081 (2005) 232–237Feng Li a, DongDong Wang a, XiuPing Yan a,?, RongGuo Sub, JinMing Lin b,?a Research Center for Analytical Sciences, College of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, Chinab Research Center for EcoEnvironmental Sciences, Academic Sinica, . Box 2871, Beijing, ChinaReceived 21 January 2005。 received in revised form 29 April 2005。 accepted 17 May 2005Available online 13 June 2005AbstractA novel method for speciation analysis of inorganic arsenic was developed by online hyphenating microchip capillary electrophoresis (chipCE) with hydride generation atomic fluorescence spectrometry (HGAFS). Baseline separation of As(III) and As(V) was achieved within 54 s by the chipCE in a 90mm long channel at 2500V using a mixture of 25 mmol l?1 H3BO3 and mmol l1 CTAB (pH ) as electrolyte buffer. The precisions (RSD, n = 5) ranged from to % for migration time, to % for peak area, and to % for peak height for the two arsenic species at mg l?1 (as As) level. The detection limits (3σ) for As(III) and As(V) based on peak height measurement were 76 and 112 μg l1(as As), respectively. The recoveries of the spikes (1 mg l1 (as As) of As(III) and As(V)) in four locally collected water samples ranged from to 106%.169。 2005 Elsevier . All rights reserved.Keywords: Speciation。 Arsenic。 Atomic fluorescence spectrometry。 Microchip capillary electrophoresis。 Miniaturization1. IntroductionArsenic is a ubiquitous trace element and it may be found in the atmosphere, water, food and in the soil. Many arsenicpounds are known to be highly toxic. Exposure to arsenic can cause a variety of adverse health effects, including dermal changes, respiratory,cardiovascular, gastrointestinal,genotoxic, mutagenic and carcinogenic effects [1]. However, the toxicity of arsenic depends strongly on its chemical forms [2]. The inorganic pounds are far more toxic than their organic metabolites [2]. Therefore, the speciation of arsenic is important for understanding both the biological and the geochemical behaviour of thiselement [1–5].The methodologies currently used for arsenic speciation mainly involve a separation technique, capillary electrophoresis (CE) [6–12] or high performance liquid chromatography (HPLC) [5,13–23], coupled with a highly sensitive elementspecific detector, such as atomic absorption spectrometry (AAS) [13–15], atomic fluorescence spectrometry (AFS) [9,10,16–19], inductively coupled plasma mass spectrometry (ICPMS) [5–8,20–23]. Microfluidic chip technology has been undergoing rapid development in recent years. It can offer many attractive advantages, including short analysis time, minimal sample/reagent consumption,portability, and eventually reduced cost [24–26]. Little work on speciation analysis with chipCE, however, has been previously reported [27–29]. ChipCE with conductivity[27] and ICPMS detection [28,29] has been employed for the determination of inorganic arsenic species. Integrateddetection in chipCE with conductivity electrodes for speciationanalysis, however, possesses poor detection and elementspecific detection techniques forchipCE speciation would provide improved performancefor this application. ChipCE coupled with ICPMS is being of growing importance in speciation analysis,and two excellent papers on this hyphenated technique for speciation have been published [28,29]. Although highlysensitive, element and isotopespecific characteristic of ICPMS makes it very attractive as an online detector for chipCE, the high instrumental and running costs of the ICPMS instrument as well as the strict requirement that the analyst be welltrained set a serious limitation on the wideapplication of such a hyphenated technique.Hydride generation atomic fluorescence spectrometry(HGAFS) has been shown to offer similar sensitivity to ICPMS for hydrideforming elements [30]. Compared with ICPMS, AFS also presents the advantages of much lower instrument and running costs, shorter warmup times prior to analysis, and easy handling [30]. The use of HGAFS as an onlinedetector of chipCE is expected t