急性創(chuàng)傷性深靜脈血栓消退與不消退差異表達基因研究doc(編輯修改稿)
2025-08-14 04:30 本頁面
【文章內(nèi)容簡介】 each point。 the clamping strength was fastening one barb of hemostatic forceps, lasting 3 seconds each time, then the incision was sutured, no drainage was set. Rat hibateral posterior limbs were fixed with hip spica casts except for group A and B. At different phases after model being produced, color and swelling extent of both feet were observed by gross observation. 3. Methods for obtaining femoral veins. Rats were anesthetized with 3% pentobarbital sodium (1ml/kg, intraperitoneal injection), supine position fixation, sterilizing anteromedial skin of hibateral posterior limbs through iodophors, exposing hibateral femoral veins. In group A, femoral vein and related main tributaries were resected. In group B, at 。 group C, at 。 group D, at 25h。 group E, F, G, at 72h after model being produced, the same region vascular tissue was also resected separately. Part of the vessel separated for HE staining pathological histological analysis, the rest were rinsed by % physiological saline to clean up blood and thrombi. The vessel specimens were put into nitrogen canister in less than 30 seconds after ex vivo, which would be used for total RNA extraction. 4. Extraction of total RNA. After affirming the status of thrombosis by histological analysis, total mRNAs of femoral vein specimens from the 7 phases were extracted separately through TRIzol method. All total RNA samples were checked by agarose gel electrophoresis and devided into two portions for being detected through genechip and RTPCR. 5. RNA detection through genechip and RTPCR. Through cRNA probes preparation, hybridization, washing, staining and scanning were performed orderly to finish array detecting according to the operation flowsheet. To validate the accuratissime of genechip through RTPCR. 6. Data screening and analysis. After performing the restrictive conditions: ① the data of experimental group in EvsA and FvsA should not be marked “absent”(that is to say, the signal intensity is weak)。 ② Signal log2 ratios of the same gene pared between the resolution group and insolution group must be ≥1 or ≤1(representing variability routinely), to search out the differential expression genes between the resolution group and insolution group. The screened genes were clustered through software of Gene Cluster and drawn visual picture. These genes functions were inquested from web “NCBI
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