培養(yǎng)基及其配制ppt課件(編輯修改稿)
2025-01-31 19:45 本頁面
【文章內容簡介】 長。 ②促進細胞分裂與擴大。 ③抑制根的分化。 生長素與細胞分裂素：它們的比例決定著發(fā)育的方向，是愈傷組織、長根還是長芽。如為了促進芽器官的分化，應除去或降低生長素的濃度，或者調整培養(yǎng)基中生長素與細胞分裂素的比例。 高：有利于根的形成和愈傷組織的形成； 適中：有利于根芽的分化； 低：有利于芽的形成 生長素 /細胞分裂素比例 No BAP BAP reduced to mg. l1 No NAA Poor shoot development and no roots. Extensive callus generation Poor shoot development and no roots NAA reduced to mg. l1 More balanced development of roots and shoots. However, undifferentiated callus is developing at the edges of the explant Profuse development of roots and a reduced number of shoots. Undifferentiated callus is developing at the edges of the explant NAA increased to mg. l1 Profuse development of roots over the explant upper and lower surface, and few shoots. Some callus No MS salts No sign of regeneration from explant at all. MS salts reduced to g. l1 Some root growth but reduced development of shoots MS salts increased to g. l1 Shoots developed on upper surface of explant. No roots or callus. GA (赤霉素 )： 有 20多種，生理活性及作用的種類、部位、效應等各有不同、培養(yǎng)基中添加的是 GA3，主要用于促進幼苗莖的伸長生長，促進不定胚發(fā)育成小植株；赤霉素和生長素協同作用，對形成層的分化有影響，當生長素／赤霉素比值高時有利于木質部分化，比值低時有利于韌皮部分化；此外，赤
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