【正文】 .............................. 7 添加回 收率試驗(yàn) ............................................................................................................. 8 3 提取與凈化方法 ................................................................................................................ 8 提取劑的選擇 .................................................................................................................. 8 提取方法與時(shí)間的選擇 ................................................................................................. 8 提取方法 ......................................................................................................................... 8 提取時(shí)間 ......................................................................................................................... 9 提取溶劑用量 的選擇 ..................................................................................................... 9 樣品凈化 ......................................................................................................................... 9 弗羅里硅土用量 ............................................................................................................... 9 洗脫劑及用量 ................................................................................................................. 9 4 結(jié)果與討論 ...................................................................................................................... 10 檢測(cè) 方法的確定 ............................................................................................................ 10 色譜柱的選擇 ............................................................................................................... 10 流速的確定 ................................................................................................................ 10 柱溫的選擇 .................................................................................................................. 10 停止和保留時(shí)間的設(shè)定 .............................................................................................. 10 波長(zhǎng)的確定 .................................................................................................................. 10 標(biāo)準(zhǔn)工作曲線 .................................................................................................................11 精確度與精密度 .............................................................................................................11 啶蟲脒 在 煙葉 中的消解動(dòng)態(tài) ........................................................................................ 12 煙葉的消解動(dòng)態(tài)結(jié)果 .................................................................................................. 12 煙草土壤的消解動(dòng)態(tài)結(jié)果 .......................................................................................... 14 5 結(jié)論 與討論 ...................................................................................................................... 16 總結(jié) ................................................................................................................................ 16 主要?jiǎng)?chuàng)新點(diǎn) .................................................................................................................... 17 參考文獻(xiàn) ................................................................................................................................ 17 致謝 ........................................................................................................................................ 19 1 70%啶蟲脒水乳劑在不同區(qū)域煙草上殘留量的研究 學(xué) 生：石建銀 指導(dǎo)老師：廖嬋娟 (湖南農(nóng)業(yè)大學(xué)資源環(huán)境學(xué)院，長(zhǎng)沙 410128) 摘 要： 本文研究并建立 啶蟲脒 在 煙草和土壤 中的殘留分析測(cè)定方法，為了了解 70%啶蟲脒水乳 劑在 煙草種植用 地施用后其有效成分 啶蟲脒 在 煙草和土壤 中的殘留消解行為，全面準(zhǔn)確地評(píng)價(jià)其生態(tài)環(huán)境的安全性，為其科學(xué)、合理 性 使用提供科學(xué)依據(jù)。當(dāng)添加質(zhì)量分?jǐn)?shù)為 — 時(shí)，啶蟲脒在煙草中的回收率為 %— %；在煙草土壤中的回收率為 %%，相對(duì)標(biāo)準(zhǔn)偏差均小于 5%。研究比較 了啶蟲脒 在湖南長(zhǎng)沙、山東青島 兩個(gè) 地 區(qū)煙草和煙草土壤 中的消解動(dòng)態(tài)，結(jié)果表明 啶蟲脒 在 煙草和煙草土壤 中的消解 動(dòng)態(tài) 規(guī)律符合一級(jí)動(dòng)力學(xué)方程 ， 半衰期為 煙草 。 關(guān)鍵詞 ： 煙草；啶蟲脒 ； 高效液相色譜法 ； 層析柱凈化法；半衰期 Study on the residual quantity of 70% acetamiprid water emulsion in different parts of tobaccon Student: Shi Jianyin Instructor: Liao Chanjuan (Hunan Agricultural University resources and Environment Institute, Changsha 410128) Abstract: In this paper, the analysis and the establishment of acetamiprid residues in tobacco and soil analysis, In order to understand 70% acetamiprid aqueous emulsion in tobacco planting after application of the effective ponents of Acetamiprid in tobacco and soil residue degradation behaviorr, Comprehensively and accurately evaluate the security of its ecological environment, and provide scientific basis for its scientific and rational this paper, the extraction of acetonitrile was extracted by acetonitrile extraction, and the purification of silica soil column in the process of analysis, HPLC 2 (HPLC) method and quantitative ana