【正文】 作用時，首次發(fā)現(xiàn)了血清中含有胰島素樣作用的促生長因子，命名為“硫化因子”[2]，因其具有擬胰島素樣活性[3]和刺激增殖活性[4]命名為生長調節(jié)素[5]，隨后被命名為IGFs[6]。IGF家族由2個生長因子(IGFI和IGFII)、IGF受體(IGFI R和IGFII R)、IGF結合蛋白(IGFBPs)及IGF蛋白水解酶組成。animal breeding), Grade 2008Supervised by Professor ZHANG Hongping Abstract:IGFBPs (insulinlike growth factors, IGFs) family play an important role in maintaining normal physical activities and processes, including embryo differentiation, body development, sugar, lipid and protein metabolism. IGFBPs can prolong the halflife of IGFs, enhance its biological activity and regulate its metabolism clearance by binding IGFs in circulation system. IGFBPs secreted from liver and other local tissues can regulate and stimulate growth of tissues corporately. In this study, Nanjiang yellow goat was choose as experimental material. RTPCR and cloning technology were employed to clone the full coding sequence of IGFBP6 genes out the total RNAs extracted from liver tissue at 0d after birth, followed by using of QPCR to identify the profile of gene expression in different tissues(liver, lung, heart, longissimusdorsi, semimembranosus, musculustriceps) and at different time points(0d、30d、60d、90d、120d after birth). The results indicate: (1) We have cloned the CDS full sequence of IGFBP6 (711bp), which encoding a peptide consists of 235 amino acid residues. The nucleotide and amino acid sequence of IGFBP6 possess high homogeneity with sheepabout 97% of its sequence as well as other spices。science (SpecialIGFBP6在背最長肌的基因表達量遠遠高于肝臟、心臟、脾臟、肺臟和其它肌肉等組織的表達量（P），在肝臟中的表達量最低。本研究以培育品種南江黃羊為試驗材料，應用RTPCR和克隆技術克隆山羊IGFBP6基因的全編碼區(qū)序列，采熒光定量PCR技術研究該基因在南江黃羊生后5個時間點（0d、30d、60d、90d、120d）和6個組織（肝臟、肺、心臟、背最長肌、半膜肌和臂三頭?。┑陌l(fā)育性變化。山羊IGFBP6基因的克隆及其組織表達的發(fā)育性變化動物科學（特種經(jīng)濟動物養(yǎng)殖方向） 2008級 游杰指導教師 張紅平 教授摘要：IGFBPs在體內循環(huán)系統(tǒng)中能與IGFs結合，延長IGFs的半衰期，調節(jié)IGFs的代射清除率，提高IGFs的生物學效應。肝臟和局部組織分泌的IGFBPs共同調控各個組織的生長與發(fā)育，從而促進動物機體的生長。結果表明：克隆得到南江黃羊IGFBP6基因的CDS全序列長度為711bp，編碼235個氨基酸，該基因的核苷酸、氨基酸序列同綿羊的一致性為97%，與其他物種間的一致性也較高；在各個時間點的各個組織中都檢測到IGFBP6基因的表達，但相對表達量存在一定的差異。關鍵詞：南江黃羊；IGFBP6基因；克??；熒光定量PCR；表達Cloning of Goat IGFBP6 Gene and its Developmental Change in Tissue ExpressionYou Jie, Animaleconomical (2)We have detected the existence of IGFBP6 mRNA in all tissues and at each time points we choose, but the expression level differ from each other. It express more higher in longissimus muscle than liver, heart, lung and other muscle tissues (P or P), while in liver, its expression reached the lowest level relatively. Key Words: Nanjiang yellow goat, IGFBP6, Clone, QPCR, Expression1 文獻綜述 IGFBPs的研究進展 IGFs簡介 胰島素樣生長因子（insulinlike growth factors,IGFs）系統(tǒng)在正常生理活動中起著極為重要的作用，其與胚胎分化、個體發(fā)育密切相關，而且參與糖、脂肪和蛋白質代謝。IGFI和IGFII結構與胰島素相似，是兩個同源性較高、分子量大約為7KDa的多肽類激素[1]。IGFs在許多組織中均有表達， IGFs除了通過旁分泌方式發(fā)揮作用外[7]，還可以在局部以自分泌的方式促進細胞增殖[8]。IGFs主要通過與IGFI受體結合發(fā)揮促進有絲分裂的作用，IGFI受體與胰島素受體結構相近，均具有受體酪氨酸激酶活性。IGFII受體對IGF的調節(jié)作用目前尚不清楚[9]。IGFs與IGFBPs的親和力高于IGFI受體，因此IGF生物活性和對IGF的代謝與IGFBPs密切相關[10]。IGFBPs是IGFs的重要組成之一，IGFBPs對于調節(jié)IGFs的生物學功能主要表現(xiàn)在以下四個方面： (1)IGFs運載蛋白的作用，并可調節(jié)血液中游離IGFs含量；(2)延長IGFs的半衰期，調節(jié)IGFs的代射清除率；(3)對特異性組織、細胞進行識別和定位；(4)調節(jié)IGFs的生物學效應，通過調節(jié)IGFs和IGFs受體的相互作用來完成；(5)不用依賴于IGFs而直接調節(jié)正常和惡性細胞的生長[1215]。IGFBPs在氨基酸結構上高度保守，在總蛋白質中有35%左右