干旱脅迫下紅麻myb轉(zhuǎn)錄因子的克隆及表達特性分析-項目立項開題報告-展示頁
2025-01-30 17:05本頁面
【正文】 有限公司)反轉(zhuǎn)錄試劑盒說明書合成cDNA 第1 鏈, –20℃保存?zhèn)溆?。并在在NCBI 的GenBank 數(shù)據(jù)庫中搜索與該基因同源的其他物種的氨基酸序列, 通過ClustalX2 軟件進行氨基酸序列比對, 進行保守結(jié)構(gòu)域分析, 軟件的NeighborJoining 算法構(gòu)建系統(tǒng)進化樹, 用BootStrap檢驗, 并重復(fù)1000 次系統(tǒng)進化分子。 由ProtScale tool ()預(yù)測蛋白質(zhì)的疏水性(金剛 et al., 2014。預(yù)期目的：獲得紅麻R2R3MYB 序列全長23條，分析其生物信息學(xué)功能，并進行原核表達驗證。：總RNA反轉(zhuǎn)錄成cDNA。：對反轉(zhuǎn)錄結(jié)果篩選R2R3MYB序列進行歸類與比對分析。參考文獻Abe H, Urao T, Ito T, Seki M, Shinozaki K and YamaguchiShinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:6378.Cominelli E, Gusmaroli G, Allegra D, Galbiati M, Wade HK, Jenkins GI and Tonelli C (2008) Expression analysis of anthocyanin regulatory genes in response to different light qualities in Arabidopsis thaliana. Journal of Plant Physiology 165:886894.Dai X, Xu Y, Ma Q, Xu W, Wang T, Xue Y and Chong K (2007) Overexpression of an R1R2R3 MYB gene, OsMYB3R2, increases tolerance to freezing, drought, and salt stress in transgenic Arabidopsis. Plant Physiology 143:17391751.Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C and Lepiniec L (2010) MYB transcription factors in Arabidopsis. Trends Plant Sci 15:573581.Ganter B and Lipsick JS (1997) Myb binding sites within the Nras promoter repress transcription. Oncogen
環(huán)評公示相關(guān)推薦
鄂ICP備17016276號-1