花生中植物螯合肽pc的研究__本科畢業(yè)論文-展示頁(yè)
2025-06-03 10:59本頁(yè)面
【正文】 指導(dǎo)教師： 完成日期： I 摘要 重金屬是我們熟知的環(huán)境污染 物之一 ， 近些年 ， 工礦業(yè)的發(fā)展，農(nóng)藥、 化肥的使用 ， 使得重金屬對(duì)水體，土壤的污染愈來(lái)愈嚴(yán)重，而在植物的器官中累積的重金屬 ，最終經(jīng)過(guò)食物鏈 迫害人類(lèi)。 利用植物減輕重金屬污染是國(guó)內(nèi)外研究開(kāi)發(fā)的熱點(diǎn)。其中， 進(jìn)入體內(nèi) 的1/3 的鎘 可被 腎臟 吸收， 腎臟 是鎘中毒的靶器官。鎘在體內(nèi)可與含 氨基、 羥基、硫基的蛋白 質(zhì)分子結(jié)合， 抑制 許多酶系統(tǒng) ，從而 使 肝、腎器官中酶系統(tǒng)的 正常功能 受阻 。 更嚴(yán)重的會(huì)使 使骨骼的代謝受阻，造成骨質(zhì)疏松、萎縮 等一系列癥狀 ，鎘的危害已經(jīng)受到人們極大地 關(guān)注 。以在不同重金屬鉻的環(huán)境下生長(zhǎng)的花生為原料，利用高效液相色譜 質(zhì)譜聯(lián)用儀來(lái)測(cè)定花生中植物螯合肽的含量。在以上的實(shí)驗(yàn)條件下 ， 檢出限 為 ； 線(xiàn)性范圍分布在 ( R2=)之間，回收率在 80% 以上，靈敏度相對(duì)較高。結(jié)果表明， 重金屬鎘污染的花生中都檢出不同濃度的植物螯合肽。 關(guān)鍵字 ： 鎘污染 ； 花生 ； 植物螯合肽（ PC） ； 高效液相色譜 質(zhì)譜 II Abstract heavy metals is known as one of environmental pollutants, in recent years, the development of industrials, the use of pesticides, fertilizers, makes the heavy metals in the aquatic and soil pollution bees more and more serious, and heavy metals are accumulated in the plant ans, the finally, human is persecuted by heavy metals through the food chain. Using plants to reduce heavy metal pollution is a hotspot of research and development at home and abroad. The study found that cadmium absorbed by the body will turn into cadmium sulfide protein, cadmium sulfide is a selective protein, can be accumulated in kidney, a third of the cadmium can be absorbed by the kidney, the kidney is the target an of cadmium poisoning. Other ans such as pancreas, spleen, thyroid gland and hair also be accumulated a certain amount. Cadmium in the body will be united by protein molecules containing amino, hydroxyl, inhibition of many enzyme system, so that the normal function of the liver and kidney ans in enzyme system will be damaged. Due to the cadmium, the patient can appear the symptom of diabetes, protein and amino acid urine, More seriously, which can make bone metabolism, cause osteoporosis, contraction and a series of symptoms, the harm of cadmium has been greatly concerned. In this study, Chelating peptide content in plants was used as an index, which explored the influence that different concentrations of cadmium induced the content of peanut plant chelating peptide, the study use peanuts grown in different heavy metal chromium as raw material and take advantage of high performance liquid chromatographymass spectrometry instrument to determine the content of peanut plant in chelating peptide. This experiment took advantage of reversed phase column C, acetonitrile and % trifluoroacetic acid, which detected the chelating peptide content of peanut under The induced of different concentrations of cadmium. In the above experimental con
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