【正文】
TUNEL assayTerminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the terminal end of nucleic acids.TUNEL is a mon method for detecting DNA fragmentation that results from apoptotic signaling cascades. The assay relies on the presence of nicks in the DNA which can be identified by terminal deoxynucleotidyl transferase, an enzyme that will catalyze the addition of dUTPs that are secondarily labeled with a marker. It may also label cells that have suffered severe DNA damage.內(nèi)容總結(jié) (1)TUNEL法檢測(cè)細(xì)胞凋亡實(shí)驗(yàn)原理和方法