tunel染色檢測細(xì)胞凋亡(文件)
【正文】 d for detecting DNA fragmentation that results from apoptotic signaling cascades. The assay relies on the presence of nicks in the DNA which can be identified by terminal deoxynucleotidyl transferase, an enzyme that will catalyze the addition of dUTPs that are secondarily labeled with a marker. It may also label cells that have suffered severe DNA damage.內(nèi)容總結(jié)
（1）TUNEL法檢測細(xì)胞凋亡實(shí)驗(yàn)原理和方法
細(xì)胞凋亡中染色體 HYPERLINK DNA的斷裂是個(gè)漸進(jìn)的分階段的過程，染色體 HYPERLINK DNA首先在內(nèi)源性的核酸水解酶的作用下降解為50300kb的大片段
。陽性對(duì)照的切片可使用DNaseI部分降解的標(biāo)本，陽性細(xì)胞對(duì)照可使用地塞米松（1μM）處理34hr的大、小鼠胸腺細(xì)胞或人外周血淋巴細(xì)胞。用蒸餾水洗3次，前兩次將載玻片提起放下10次，最后1次靜置30s。用PBS洗4次，每
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