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( 1 7 )Ne g a t iv e r e g u la t io n o f g e n e e x p r e s s io n ( 1 3 )An a t o m ic a l s t r u c t u r e m o r p h o g e n e s is ( 1 2 )DNA m e t a b o lic p r o c e s s ( 2 6 )Re g u la t io n o f t r a n s c r i p t io n , DNA d e p e n d e n t ( 6 3 )Ce ll d i v is io n ( 2 2 )Re s p o n s e t o e t h y le n e s t im u lu s ( 1 2 )F r u it d e v e l o p m e n t ( 1 2 )Re s p o n s e t o c o ld ( 1 1 )Ne g a t iv e r e g u la t io n o f d e v e lo p m e n t a l p r o c e s s ( 1 1 )Re g u la t io n o f b io lo g ic a l q u a lit y ( 1 9 )O x id a t io n r e d u c t io n p r o c e s s ( 2 2 )O r g a n d e v e lo p m e n t ( 2 4 )Re s p o n s e t o lig h t s t im u lu s ( 1 3 )Re s p o n s e t o o t h e r o r g a n is m ( 1 1 )Re s p o n s e t o a b s c is ic a c id s t im u lu s ( 1 1 )Nu c le o s o m e a s s e m b ly ( 2 3 )Ce ll c y c le p r o c e s s ( 1 5 )De f e n s e r e s p o n s e ( 1 5 )His t o n e ly s in e m e t h y la t io n ( 1 2 )Ce ll u la r r e s p o n s e t o s t r e s s ( 1 4 )Re g u la t io n o f g e n e e x p r e s s io n , e p ig e n e t ic ( 1 3 )Re s p o n s e t o w a t e r d e p r iv a t io n ( 1 5 )Re s p o n s e t o o s m o t ic s t r e s s ( 1 1 )Re s p o n s e t o a u x in s t im u l u s ( 1 3 )Im m u n e s y s t e m p r o c e s s ( 1 2 )Ne g a t iv e r e g u la t io n o f c e llu la r p r o c e s s ( 1 6 )Ce ll d i f f e r e n t ia t i o n ( 1 3 )Ho r m o n e m e d ia t e d s i g n a l in g p a t h w a y ( 1 7 )Re g u la t io n o f c e ll c y c le ( 1 7 )RNASeq: Transcriptional profiling Vernalization and flowering induction in Arabidopsis DAMs Flower bud development Winter Chilling temperatureSOC1 FT AP1? LFY AG, AP3, PI, SEP Flower induction SVP Cool temperature FLC FRI Fall Winter Vernalization Histone methylation Epigeic programming Dormancy and flower development in peach Meiosis amp。 division Stamen amp。 carpel, ovule Genes differentially regulated during the chilling treatment CRC WUS ppa014900m (CRC) LFY and CRC appear to be regulated by DNA methylation CRC WUS DAM5 DAM3 DAM4 T1 T2 T3 D3 T4 DAM6 CRC WUS Genomewide ChIP analysis: Detection of trimethylation of histone 3 K27 at DAM5 and DAM6 chromatin during dormancy release Vernalization and flowering induction in Arabidopsis DAM5 amp。6 Mature flower bud Winter Chilling temperatureSOC1 FT AP1? LFY AG, AP3, PI, SEP Flower induction SVP Cool temperature FLC FRI Fall Winter Vernalization Histone methylation H3K27me3 Chilling temperature and flower development LFY DNA methylation SOC1 AG,SEP WUS, CRC Regulatory mechanism Dormant buds 0 (H) 500 1000 D3 D7 D14 D A M 5 amp。 6 Chromatin and DNA methylation pathways: Two separate but related epigeic programming How does the epigenome looks like? FT SOC1 LFY AP1 FLC, a key flower repression gene targeted by vernalization in Arabidopsis