產(chǎn)植酸酶黑曲霉菌株s8-的基因擴(kuò)增與鑒定-資料下載頁(yè)
2025-08-04 13:31本頁(yè)面
【正文】 od research international .2022,34(8). 715720.[7] Piddington C S,Paloheimo M,et clong and sequencing of the genes encoding phytase(phy) and pH acid phosphatase(aph) from Aspergillus niger var awamori. Gene, 1993, 133:5562[8] Van Hartingsveldt M, Van Zeijl C M J, Hartveld G M,et al. Clong, chatacterization and overexpression of the phytaseencoding gene(phyA) of Aspergillus niger. Gene, 1993,127:8794AMPLIFING AND IDENDIFING OF THE PHYTASE PHYA GENE OF ASPERGILLUS NIGER S8Zeng Xiu1 Guo Wanzhu2 Kong Lujun1 Wang Xiaoyou1(1. Chongqing Swine Science Academy， Chongqing， Rongchang， 402460。(2. Sichuan Agricultrural University Animal Technology Academy,Sichuan Ya’an,625014)Abstract:The thesis mainly reported that: in the three methods used to extract genome DNA from S8 Aspergillus niger mycelium, the best was modified Vitagine Kit. In the two pairs of primers selected by software and one pare of primer reported as parision, the best primer was not got rid of the intron of 5PhyA gene and brought KPnⅠand XbalⅠenzyme digestion site，and the length of the PCR product was about . In the meantime,the system of PCR was optimized. The best result of purifing and recovering the objective fragment was PCR Fragment Recovery Kit in the three varied methods，and the output could attain 10ng/ to the reports about the enzyme digestion site of the phyA gene,the objective fragment could be digested by BamHⅠ、Sal Ⅰ and couldn’t by HindⅢ、XbaⅠ、KPnⅠ.This result agreed with the known enzyme ,we could go step further to judge that the PCR product had phyA gene.KEY WORDS: phyA，PCR，phyA gene，enzyme digestion analyse
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