mir396在豆科植物苜蓿根中對(duì)其菌根化和分生組織活性的影響-資料下載頁(yè)
2025-05-07 18:10本頁(yè)面
【正文】 反應(yīng)，并且GRF5在 BR處理下下調(diào)，說(shuō)明BR和 miR396b, GRF5之間有某種作用關(guān)系 2022/6/4 29 Levels of GUS transcript in roots expressing the prommiR396b:GUS construct (grey bars) or the promGRF5:GUS construct (black bars) treated or not treated with 1 nM BR for 3 h were measured using quantitative RTPCR. BR可以活化miR396b的啟動(dòng)子活性，但 promGRF5:GUS并沒(méi)有明顯減少，說(shuō)明 BR可能是在轉(zhuǎn)錄后水平抑制 GRF5基因的表達(dá)，而且 BR和miR396b存在潛在聯(lián)系，但本文中沒(méi)有對(duì)其研究 ? miR396 limits mycorrhizal colonization but not nodulation ? We next decided to study the potential role of miR396 in root symbioses. ? We analysed mtrmiR396a and mtrmiR396b expression profiles in roots inoculated with the symbiotic bacteria Sinorhizobium meliloti. 2022/6/4 30 2022/6/4 31 miR396a啟動(dòng)子首先在根瘤導(dǎo)管組織中發(fā)現(xiàn)， miR396b啟動(dòng)子在維管組織，感染區(qū)（ Ⅱ ）和氮固定區(qū)（ Ⅲ ）含量很高 2022/6/4 32 雖然 miR396存在于根瘤中，但 miR396OE和 MIM396對(duì)根瘤密度都沒(méi)有明顯影響 ? We also analysed the expression of miR396 in response to MycLCOs, the lipochitooligosaccharide signal molecules produced by AM fungi. ? The involvement of miR396 during mycorrhization was then analysed at 5 weeks after colonization with R. irregularis (formerly Glomus intraradices) in miR396OE and MIM396 roots. 2022/6/4 33 2022/6/4 34 miR396 affects fungal colonization during AM symbiosis. 菌根的叢枝量和形態(tài)沒(méi)變化，但 AM定植在miR396OE根中的量減少，而在 MIM396中的量增加 Hence, the action of miR396 limits mycorrhizal colonization. ? Here, we showed that, in the model legume M. truncatula, miR396 represses not only MtGRF but also two MtbHLH79 TFencoding genes, and that its deregulation affects root growth and the ability to be colonized by mycorrhizal fungi. 2022/6/4 35 2022/6/4 36
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