embryoidbodiesfromembryonicstemcellsin-vitro：胚狀體從胚胎干細胞體外培養(yǎng)-資料下載頁
2025-01-12 07:57本頁面
【正文】 and RTPCR analyses demonstrated that endodermal cell differentiation occurred during the EB development ?Two types of populations were expressed by day 5 ?Yolksaclike structures ?Hematopoietic cells ?The TTR messages were found only in the population of the EBs which began to form yolksaclike structures and were only found in the outer endodermal layer of the yolksaclike structures This suggests that at least one of the endoderm marker genes is predominantly expressed in the outer layer of the yolksaclike structure during EB development The order of gene expression for the in vivo studies is similar to that found in EB formation in vitro Conclusion ?These data as a whole strongly suggest that development of EBs in vitro closely resembles the sequence of in vivo (normal) development of visceral yolk sac endoderm. ?The ES cell in vitro differentiation system should be useful for analyzing molecular events concerning both extraembryonic and embryonic endoderm differentiation processes The End
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鄂ICP備17016276號-1