embryoidbodiesfromembryonicstemcellsin-vitro：胚狀體從胚胎干細(xì)胞體外培養(yǎng)(已修改)
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【正文】 Embryoid Bodies From Embryonic Stem Cells Invitro Robert Christensen Biology Department Eastern Connecticut State University Endoderm–Specific Gene Expression in Embryonic Stem–Cells Differentiated To Embryoid Bodies Experimental Cell Research 229 (1999): pp 2734 Koichiro Abe, Hitoshi Niwa, Katsuro Iwase, Masaki Takiguchi, Masataka Mori, Shin– Ichi Abe, Kuniya Abe, and Ken Ichi Yamamura Introduction ? Cell lineages arise during development ?Ectodermal tissues ?Mesodermal tissues ?Endodermal tissues ? EBs resemble the embryo of the egg cylinder stage ?Neuronal cells ?Cardiac muscle cells ?Hematopoietic cells ?Yolk sac cells ? ES cells have full developmental potential ? EBs from ES cells ? EBs consist of: ? Later stages EBs are posed of: ?Past studies showed specific changes in expression of endoderm marker genes during EB development and suggested that EB formation could be considered an invitro model for endoderm differentiation. ?RNA blot ?Reverse transcri
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