embryoidbodiesfromembryonicstemcellsin-vitro：胚狀體從胚胎干細(xì)胞體外培養(yǎng)(參考版)
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【正文】 sectioning of EBs ?These experiments were carried out as described by Sasaki and Hogan ?Stained EBs were refixed in paraformaldehyde and glutaraldahyde for 30 minutes ?Then washed twice with PPT and placed in molten agarose, solidified, then sectioned Molecular Probes ?Xho I fragment of R1 used as ?fetoprotein (AFP) probe ?A fragment of Sma I – Sac I fragment of used as a hepatocyte nuclear factor (HNF) probe ?A 660bp Pst I – Pvu II fragment of pmPA1 was subcloned and used as a transthyretin (TTR) cDNA probe ?A variant form of HNF1 (vHNF1) probe was synthesized by PCR using adult mouse liver cDNA as a template ?Serum albumen (ALB) probe was also made by PCR Results ?vHNF1 and HNF4 transcript levels were very low in the undifferentiated, preaggregation and day 0 EBs, then abruptly incresed at day 1 ?HNF3? began weak, but the expression level increased after day 1 ?TTR expression was still low at day 1, but increased rapidly thereafter ?These results demonstrate that the activation of vHNF1, and HNF4
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