考研資料北京大學(xué)細(xì)胞生物學(xué)第六章基質(zhì)與內(nèi)膜(上)-資料下載頁
2025-01-07 15:24本頁面
【正文】 ation model and vesicular transport model Two possible models explaining the anization of the Golgi plex and the transport from one cisterna to the next. 十 十十C. Golgi Biogenesis Stages of Golgi growth and division. Shown are thin section electron micrographs of T. gondii RH tachyzoites replicating by endodyogeny in HFF cells. Cells were placed in one of four categories according to the number and size of the Golgi: a, single Golgi。 b, single, elongated Golgi。 c, two Golgi。 d, Golgi, often more vesiculated, in each nascent daughter cell, delineated by the growing inner membrane plex (IMC). a, apicoplast。 dg, dense granules。 er, ER。 es, ER exit sites on the outer flattened part of the nuclear envelope。 G, Golgi。 m, micronemes。 mit, mitochondria。 r, rhoptries. Scale bar, . Stable expression of mammalian Golgi proteins. a, b, Overlaid immunofluorescence and phase images of GRASP–YFP (a) and NAGTI–YFP (b) in stable, transgenic cell lines of Toxoplasma gondii. c–h, Immunofluorescence images of a transgenic cell line expressing both GRASP–CFP (green) and NAGTI–YFP (red) before (c–e) or after (f–h) treatment with 5mg /ml BFA for 10 min at 37186。C. Merged images are shown on the right. Asterisks indicate a secreted form of NAGTI–YFP that accumulates in the parasitophorous vacuole. Scale bars, 5mm. Immunoelectron microscopy of transgenic parasites. a–c, Cryosections of GRASP–YFP (a, c) or NAGTI–YFP (b) transgenic parasites, pretreated for 2 h with 50mg/ml cycloheximide, before being fixed and immunolabelled for YFP using polyclonal antibodies against GFP followed by protein A coupled to 5nm gold particles. Note the high density of labelling restricted to Golgi membranes. In c, GRASP–YFP transgenic parasites were treated with BFA (5mg/ml) for 30 min before immunolabelling. Note the tubulovesicular appearance of the Golgi caused by loss of Golgi enzymes to the ER. d, Quantification of images in a and b. Results are presented as mean 177。 . gold particles /um2.
環(huán)評公示相關(guān)推薦
鄂ICP備17016276號-1