中南大學(xué)中藥現(xiàn)代化研究中心(編輯修改稿)
2025-01-23 10:59 本頁(yè)面
【文章內(nèi)容簡(jiǎn)介】 Figure’TheamplifiedtotalioncurrentchromatogramoffractionN6obtainedfromGCMS. 定性分析 用質(zhì)譜庫(kù)相似檢索定性，結(jié)合色譜保留指數(shù)確正質(zhì)譜的定性結(jié)果 色譜峰 B分辨所得六種物質(zhì)定性，它們依次是 ：6methoxy2methylbetacarboline(C13H12N2O)、3,3’dimethyl[1,1’biphenyl]4,4’diamine(C14H16N2)、2,5dimethyl4[3amino4methylphenyl]pyridine(C14H16N2)5acetyl4amino2methylthiothiophene(C8H8N2OS2)、4,9dimethylnaphtho[2,3b]thiophene(C14H12S)、1,2,5,6tetramethylacenaphthylene(C16H16)依此原理，分辨七個(gè)餾分的所有色譜峰，對(duì)各個(gè)組分進(jìn)行定性分析。在 1200多組分中鑒別出 130多種含氮化合物 化學(xué)計(jì)量學(xué)與中藥現(xiàn)代化研究中草藥的現(xiàn)代化與國(guó)際化是勢(shì)不可擋的世界潮流l 美國(guó)自 30到 60年代，幾乎停止植物藥的研究（ Interest in medical plants progressing significantly diminished in the 1930s and botancals fell into almost plete disuse until 1960s, Roy Upton, Herbalist Executive Director, American Herbal Pharmacopoeia） ， 到現(xiàn)在 （ 2023） Global Herbal Market : US$ billion !!（196個(gè)億美金）；德國(guó)和法國(guó)聯(lián)合開(kāi)發(fā)銀杏葉提取物 EG6761，找到最佳銀杏提取物的指紋圖譜，導(dǎo)致植物藥的在歐美重新流行Health Products (Western Countries)Ginggo Biloba (銀杏 ) St. Jone’s Wort(貫頁(yè)連翹 ) Health Products (Western Countries)Green tea(綠茶 ) Echinacea(紫錐花 ) Traditional Chinese Medicine (TCM) Health ProductsDong Quai(當(dāng)歸 ) Ginseng(人參 ) Traditional Chinese Medicine (TCM) Health ProductsCordyceps(冬蟲(chóng)夏草 ) Galic(大蒜 ) Natural Product Marketl Global Herbal Market 2023 : US$ billion !! Natural Product Market缺乏適當(dāng)或可接受的評(píng)價(jià)傳統(tǒng)草藥的研究方法Despite its existence and continued use over many centuries, and its popularity and extensive use during the last decade, traditional medicine has not been officially recognized in most countries. … The quantity and quality of the safety and efficacy data on traditional medicine are far from sufficient to meet the criteria needed to support its use worldwide. The reasons for the lack of research data are due not only to health care policies, but also to a lack of adequate or accepted research methodology for evaluating traditional medicine.傳統(tǒng)植物化學(xué)方法研究中藥的窘境l 每個(gè)化學(xué)物質(zhì)都分離了（耗時(shí)），但藥效似乎都不強(qiáng)，找到活性單體藥無(wú)法與同類(lèi)西藥相比；l 中藥的君臣佐使，協(xié)同作用難以體現(xiàn)；l 日本人、德國(guó)人（銀杏）的發(fā)現(xiàn)，指紋圖譜的提出；l 中藥現(xiàn)代化需要新思路，新方法 。中草藥現(xiàn)代化與國(guó)際化研究中的幾個(gè)難點(diǎn)問(wèn)題– 中草藥的化學(xué)基礎(chǔ) 。(多組分復(fù)雜混合體系 )– 中藥復(fù)方制劑 。(“ 膏丹丸散 ,神仙難辯 ”)。–中藥國(guó)際化的主要難點(diǎn)：中藥基地軌范化 (GAP 和 GEP)，中藥生產(chǎn)標(biāo)準(zhǔn)化 (GMP)， 基于化學(xué)指紋圖譜的中藥質(zhì)量控制 。–中藥作用機(jī)制研究 （君臣佐使 ,協(xié)同作用）– 中藥藥效及藥代動(dòng)力學(xué)方法的建立 。– 中藥譜 （化學(xué)指紋圖譜） 效 （藥理藥效） 學(xué)的創(chuàng)立 。– 中醫(yī)藥理論科學(xué)化的艱巨任務(wù)廣西玉林廣東肇慶越南云南中藥指紋圖譜在中藥現(xiàn)代化研究中的核心地位Essential Constituents of Ramulus Cinnamomi(桂枝） 中藥現(xiàn)代化的機(jī)遇與挑戰(zhàn)l基于中藥指紋圖譜的質(zhì)量控制、l基于中藥指紋圖譜的藥效研究、l基于中藥指紋圖譜的藥代動(dòng)力學(xué)研究、l餾分組合（雞尾酒方法），新型更有效復(fù)方制劑的發(fā)現(xiàn)與開(kāi)發(fā)、l全 依賴(lài)于中藥指紋圖譜的快速定性定量解析！中藥色譜指紋圖譜的測(cè)定方法以解決中藥長(zhǎng)期處于 “丸散膏丹，神仙難辨 ”的被動(dòng)局面Instruments Commonly used for Chemical Analysis of Chinese Medicinel Thin Layer Chromatography (TLC)(薄層色譜 )? qualitative analysis semiquantitative analysisl High Performance Liquid Chromatography (HPLC)(高效液相色譜 )? Both qualitative analysis quantitative analysisl Gas Chromatography (GC)(氣相色譜 )? Both qualitative analysis quantitative analysisl DNA Analysis( DNA分析 )l Capillary Electrophoresis (CE) (毛細(xì)管電泳 )中藥指紋圖譜剖析的新工具聯(lián)用色譜儀器Hyphenated Instrument (聯(lián)用儀器 )l Most modern and advanced analytical instrument.. HPLCDAD, GCMS, CEDAD, etc.l Combination of two or more instruments. HPLC DAD (diodearray detector)GC MS (Massspectrometer)Separation InstrumentSignalInstrumentl Get more data/informationHPLC DAD3D chromatogramHPLC chromatogram of nuclueside of Cordyceps Sinensis (冬蟲(chóng)草 ) at one wavelengthHyphenated Instrument (聯(lián)用儀器 )Hyphenated Instrument (聯(lián)用儀 器 )GC instrument GC chromatogram of peptic powder (平胃散 )Mass spectrum taken at retention time minutesHyphenated Instrument (聯(lián)用儀 器 )Advantages :l More data is obtained at a retention time.l More spectral information acquired to give three dimensional (3D) data.l Much more information available to analyze plicated system like TCM.Hyphenated Instrument (聯(lián)用儀 器 )Disadvantages :l Huge amount of 3D data is obtained.l Need data processing methods for information extraction, pattern recognition, etc.l Computer power was poor before. Usual practice : Use a few data obtained from HPLCDAD/GCMS to find marker ponents or active ingredients. Now, everything bees possible with high tec
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