【文章內(nèi)容簡介】 imally。 and 0, left forelimb did not move.Julio H. Garcia, MD。 Simone Wagner, MD。 KaiFeng Liu, MD。 Xiaojiang Hu, MD, PhD。Neurological Deficit and Extent of Neuronal Necrosis Attributable to Middle Cerebral Artery Occlusion in Rats。Stroke. 1995。26:627635。16．ClimbingThe rat was placed on the wall of a wire cage. Normally the rat uses all four limbs to climb up the wall. When the rat was removed from the wire cage by pulling it off by the tail, the strength of attachment was noted. Scores indicate the following: 3, rat climbed easily and gripped tightly to the wire。 2, left side was impaired while climbing or did not grip as hard as the right side。 and 1, rat failed to climb or tended to circle instead of climbing17. Body ProprioceptionThe rat was touched with a blunt stick on each side of the body, and the reaction to the stimulus was observed. Scores indicate the following: 3, rat reacted by turning head and was equally startled by the stimulus on both sides。 2, rat reacted slowly to stimulus on left side。 and 1, rat did not respond to the stimulus placed on the left side.18. Response to Vibrissae TouchA blunt stick was brushed against the vibrissae on each side。 the stick was moved toward the whiskers from the rear of the animal to avoid entering the visual fields. Scores indicate the following: 3, rat reacted by turning head or was equally startled by the stimulus on both sides。 2, rat reacted slowly to stimulus on left side。 and 1, rat did not respond to stimulus on the left side.改善記憶作用1．跳臺試驗跳臺裝置為lOcm X 15cm X 60cm的被動條件反射箱，四周用黑色塑料板、底面鋪可通電的銅柵，每間內(nèi)放置一高4 .5cm，先將動物放在反應(yīng)箱內(nèi)的橡皮臺上適應(yīng)3min，然后立即通以40V交流電，動物受到電擊后逃避反應(yīng)為跳上橡皮臺。記錄5min內(nèi)大鼠受到電擊次數(shù)，稱為錯誤次數(shù)。24h后在底部銅柵通電情況下，直接將動物置于平臺上，記錄第1次跳下的潛伏期和5 min內(nèi)的錯誤次數(shù)。2．避暗實驗 Avoidance response根據(jù)大鼠有嗜暗習性分設(shè)明暗兩室。明室上方20cm處懸一個60 W日光燈，在暗室的中后部分底部銅柵加40 V電壓，兩室之間設(shè)有一直徑為3cm大小的圓洞。實驗時將大鼠背對洞口放入明室，同時啟動計時表，動物自放入明室至進入暗室遭電擊所需時間為潛伏期，鼠受電擊后可自行逃出暗室。記錄潛伏期及5min內(nèi)動物受電擊次數(shù)，24h后復(fù)測。4．水迷宮試驗（Morris ） (24士2)℃. 。試驗包括:①定位航行試驗(place navigation test):實驗歷時7d。 。 s。將大鼠從平臺上拿卜來休息60 。②空間探索試驗(spatial probe test) s內(nèi)其在平臺象限的滯留時間。 水迷宮試驗主要用于測試大鼠對空間位置覺和方位覺的學習記憶能力[5]水迷宮試驗測試的一個重要指標是大鼠的平均逃避潛伏期平均逃避潛伏期指的是大鼠在放入迷宮后學會在5中內(nèi)找到平臺所需要的平均時間,主要與海馬功能有關(guān),與大腦皮層也有一定的關(guān)系。Morris Water MazeThis is a task that measures learning and memory in an aqueous environment. Briefly, the rat was placed in a 4ftdiameter water tank that is visually separated into four quadrants. In the center of one quadrant, the goal quadrant, a platform is hidden 1 inch below the waterline. The rat was placed in the tank at one of four start locations (north, south, east, west) and swam for up to 60 seconds until it found the platform. Once the animal located the platform, it remained on it for 20 seconds. In the event that an animal did not locate the platform, the observer positioned the animal on the platform at the end of the 60second swim period. During a 2 to 4minute intertrial interval, the animals were kept warm by infrared heating lamps. The temperature of the water maze was 20177。176。C. The latency and path length of the rat were measured by a video tracking system interfaced to a puter.Nancy E. Alexis, MS。 W. Dalton Dietrich, PhD。 Edward J. Green, PhD。 Ricardo Prado, MD。 Brant D. Watson, PhD. Nonocclusive Common Carotid Artery Thrombosis in the Rat Results in Reversible Sensorimotor and Cognitive Behavioral Deficits. Stroke. 1995。26:23382346. Y迷宮學習記憶訓練及測試:。 mm。電流強度0. 7 。 II IIII臂順序輪流作為安全區(qū)。大鼠在起步區(qū)靜置3 m 。學習訓練及測試:規(guī)定大鼠受電擊后從起步區(qū)直接逃至安全區(qū)為“正確反應(yīng)”.以達到連續(xù)10次電擊均為正確反應(yīng)(即10/10)前所需的電擊次數(shù)(即嘗試次數(shù))表示其學習獲得能力(acquisition)。記憶再現(xiàn)(retrieval)測試:選上述達到10/ 。Motor behavior(1) observation of spo