實驗二pcr乙肝rapd(編輯修改稿)
2025-02-02 02:28 本頁面
【文章內容簡介】 n genome. ? In 1983, Kary Mullis invented the PCR. The process is an invaluable tool to today39。s molecular biologists and biotechnology corporations. Kary B. Mullis As he wrote later in Scientific American: Beginning with a single molecule of the geic material DNA, the PCR can generate 100 billion similar molecules in an afternoon. The reaction is easy to execute. It requires no more than a test tube, a few simple reagents and a source of heat. (1944 ) The Nobel Prize in Chemistry 1993 for his invention of the polymerase chain reaction (PCR) method（ 1/2 of the prize ） 3 step and 30 cycles for PCR Step 1. Denaturation Step 2. Anneal Step 3. Extend 30 cycles create over a billion copies of the sequence Step 1. Denaturation ? The reaction is first heated to denature (separate) the sides of the double stranded DNA Temperature: ~ 95 degrees Celsius Step 2. Anneal ? then cooled to allow the primers to find and bind to their plementary sequences on the separated strands. Temperature: ~ 55 degrees Celsius Step 3. Extend ? the polymerase to extend the
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