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Copyright 169。 2022 Pearson Education, Inc. publishing as Benjamin Cummings Anticipatory Questions ? 1. What might happen if an anism had its cells expressing all genes within the genome all the time? ? 2. At what levels can control of cellular activities/pathways be controlled? ? 3. Based on our discussions up to this point, what do you think the term “negative feedback” means? ? 4. What steps are involved in the initiation of prokaryotic transcription? Copyright 169。 2022 Pearson Education, Inc. publishing as Benjamin Cummings Learning Objectives ? understand that regulation of gene expression is a means by which to control timing and rate of generation regarding functional gene product (either RNA or polypeptide/protein). ? explain the concept of an operon in terms of ponents’ functions (promoter, operator, repressor, corepressor, inducer, gene cluster, polycistronic transcript). ? pare and contrast repressible and inducible operon systems/pathways. ? pare and contrast negative versus positive regulation of operons ? apply the operon concept to gene expression as it relates to geic engineering (specifically, our cloning and expression of the “tomato” gene). Copyright 169。 2022 Pearson Education, Inc. publishing as Benjamin Cummings Individual bacteria respond to environmental change by regulating their gene expression ? A bacterium can