【正文】
Polycistronic mRNA transcription translation translation translation translation 。 2022 Pearson Education, Inc. publishing as Benjamin Cummings araC regulatory gene repressor Inducer (arabinose) Gene D Arabinose operon with inframe foreign DNA inserted: Tomato gene start start start start stop stop stop stop Protein B Protein A Protein D Red Fluorescent Protein (RFP) Gene B Gene A Gene D Tomato gene 5180。 Positive Regulation a) In the presence of arabinose: ?CAPcAMP plex and araCarabinose plex bind to initiator region ?this allows RNA polymerase to bind to the promoter ?transcription begins b) In the absence of arabinose: ?araC protein assumes a different conformation ?acts as a repressor ?binds to araI and a second operator region araO ?forms a loop ?this loop prevents transcription Copyright 169。 2022 Pearson Education, Inc. publishing as Benjamin Cummings Catabolite Activator Protein Mechanism ? 8/ ? Click on “bination of switches the lac operon” Copyright 169。 2022 Pearson Education, Inc. publishing as Benjamin Cummings ? Inducible enzymes usually function in catabolic pathways ? Repressible enzymes usually function in anabolic pathways ? Regulation of the trp and lac operons involves negative control of genes because operons are switched off by the active form of the repressor Copyright 169。