微生物培養(yǎng)基culturalmedium-文庫吧資料
2025-01-05 00:52本頁面
【正文】 NH4)2SO4 ?2NH3 + H2SO4 NaNO3 + 4H2 ? NH3 + 2H2O +NaOH ? 生理酸性物質(zhì) ： NH4 ?酸性物，硫酸銨 ? 生理堿性物質(zhì)： NH4 ?堿性物，硝酸銨 ? 正確使用 生理酸堿性物質(zhì) ? Except using as a nitrogen source, ammonia is often used to adjust pH of media. Stirring is necessary. 無機(jī)鹽及微量元素 mineral salts and trace elements ? Mineral salts and trace elements are needed when microbial cells grow and reproduce. ? Most mon mineral salts and trace elements: 磷 (phosphorus)、鎂 (magnesium)、硫 (sulphur)、鉀 (potassium)、鈉 (sodium)、鐵 (iron)、氯(chlorine)、錳 (manganese)、鋅 (zinc)、鈷(cobalt)、 鈣等 ? 作為微生物細(xì)胞生理活性物質(zhì)組成或生理活性作用的調(diào)節(jié)物。 cheaper, often used in production of penicillin and glutamic acid. ? yeast extract ? peptones ? 有機(jī)氮源除了作為菌體生長繁殖的營養(yǎng)外，有的還是產(chǎn)物的前體。如纈氨酸可提高紅霉素的發(fā)酵單位。 anic nitrogen sources ? microbial proteinase ?amino acids ?metabolized by microbes. ? anic nitrogen sources contain rich proteins, peptides, amino acids, vitamins and growth factors. ? 在含有機(jī)碳源的培養(yǎng)基中，微生物常表現(xiàn)出生長旺盛、菌絲或細(xì)胞濃度增長迅速的特點。 氮源 nitrogen sources ? 氮源主要用于構(gòu)成細(xì)胞物質(zhì)（氨基酸、蛋白質(zhì)、核酸等）和含氮代謝物。 糖類 sugars ? Sugars used mainly: glucose, molasses (糖蜜 ), dextrin (糊精 ). ? Glucose: most microbes can utilize it. 速效碳源、 high concentration?speed respiration up?DO no enough?intermediates (中間產(chǎn)物）accumulation ? inhibiting growth ? molasses (糖蜜 ) ? Beet(甜菜 ) and cane molasses are byproducts of the sugar industry. ? containing 50% 75% fermentable sugars, mainly sucrose. Also containing nitrogenous pounds, vitamins and minerals. ? Cheaper Starch and dextrin (糊精 ) ? Starch: widely used in fermentation industry starch ? hydrolyze using microbial amylase ?dextrin ?glucose Most mon used starches: corn, wheat, potato starches. Also using corn flour. advantages: 緩效碳源、 cheaper than glucose 油和脂肪 oils and fats ? Oil and fats are also used as carbon sources because some microbes contain lipase (脂肪酶 ) ? Fat ?glycerol(甘油 ) and fatty acids ? oxidize ? CO2+ water, releasing much energy ? Providing enough O2 is important. ? 常用的油：豆油、菜子油、豬油、魚油、棉子油等。 the ponents for synthesis of products. ? These can include simple sugars, plex carbohydrates, alcohols, amino and other anic acids, and shortchain lipids. 常用的碳源有糖類、油脂、有機(jī)酸和低碳醇。 ? 配制要求：其組成除有菌體生長所必需的元素和化合物外，還需有合成產(chǎn)物所需的特定元素、前體和促進(jìn)劑等。 ? 發(fā)酵培養(yǎng)基 production media 發(fā)酵培養(yǎng)基 是供菌體生長、繁殖和合成產(chǎn)物之用。 ? 種子培養(yǎng)基 inoculum media: 種子培養(yǎng)基 是供孢子發(fā)芽、生長和大量繁殖菌絲體，并使菌絲體長得粗壯，成為活力強(qiáng)的‘種子’。 ? 大米和小米常用作霉菌孢子培養(yǎng)基，因它們含氮少、疏松、表面積大，是較好的孢子培養(yǎng)基。 ? 按用途分類 孢子培養(yǎng)基、種子培養(yǎng)基和發(fā)酵培養(yǎng)基 spore media 要求：使菌體迅速生長，產(chǎn)生較多的優(yōu)質(zhì)孢子，不易引起菌種發(fā)生變異 (vari
黨政相關(guān)相關(guān)推薦
鄂ICP備17016276號-1