科大高分子課件四聚合物在生物高分子分離中的應(yīng)用-文庫吧資料
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【正文】 3. 多功能分離介質(zhì)的合成及應(yīng)用研究 (1) HECgPDMA 研究背景 能進(jìn)行 DNA、蛋白質(zhì)、氨基酸、同分異構(gòu)體等的分離 14 15 16 17 18 19 20 21 22 23 24 25 26 2713 14 15 16 17 18 19 20 21 22 23 24 25 2612 13 14 15 16 17 18 19 20 21 22 23 24 2510782343102711941181353281872603 M i g r a t i o n t i me ( mi n )c721353107887260331028127123419472118 Intensityb13531078872603281271310234194118 a+Separation of Φ 174/HaeIII digest by CE with HECgPDMA at (a) % w/v。 Tf, transferrin。 α2M, α2macroglobulin。 temperature 25℃ . Effect of buffer pH on the separation of basic proteins. separations were taken in PEO113bP4VP294 coated capillary Electropherograms of plasma sample in bare capillary (A) and a PEO113bP4VP294 coated capillary (B). Separation buffer: 19mM NaOHNa2B4O7 at pH . HSA, human serum albumin。 500V/cm。 3, ribonuclease. (A) Typical TEM images of (a) PEO113bP4VP45, (b) PEO113bP4VP90, (c) PEO113bP4VP113, and (d) PEO113bP4VP294。 3 = Ribonuclease A. ２．結(jié)構(gòu)規(guī)整的聚合物 PEObP4VP N O O B r O O O O N N + B r samples Mn 1H NMRa) ( 104) Mn， GPCb) ( 104) Mw/Mn (GPC) PEO113bP4VP45 PEO113bP4VP90 PEO113bP4VP113 PEO113bP4VP294 a. Mn of PEObP4VP estimated by 1H NMR； b. Mn of PEObP4VP determined by GPC. Formation of the PEObP4VP 1H NMR and GPC data of PEObP4VP copolymers Effect of molecular weight of P4VP block on the separation of basic proteins at pH . 1, lysozyme。 3 = Ribonuclease A. Migration time reproducibility (n = 3) and peak efficiency of proteins separated in polymercoated capillaries at pH (２ ) HECgP4VP OOOO HH OO O HO H O O O HOO HOH E C =H E C + C H 2 = C HN( N H 4 ) 2 C e ( N O 3 ) 6H E CNC H 2 C H( ) n2 4 6 8
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