科大高分子課件四聚合物在生物高分子分離中的應(yīng)用-文庫吧
2025-01-01 20:26 本頁面
【正文】 ( C H 3 ) 3 C l OOOO HH OO O HO H O O O HOO HO OOOO HH OOO H O O O HOO HOON +C l C H 3C H 3C H 3H OFormation of the catHEC Schematic diagram of adsorption of catHEC onto silica surface. (1) CatHEC No. HEC (g) Glycidyltrimethylamminium chloride (g) Viscosity () Nitrogen content (%) CatHEC1 380 CatHEC2 375 CatHEC3 370 Preparation and properties of CatHEC 0 2 4 6 8 10pH= 2. 2M igr at ion tim e (m in)123(a)0 2 4 6 8 10 12 14pH=M igration time (min)123(b)0 2 4 6 8 10 12 14pH=M igration time (min)123(c)2 4 6 8 10 12 14pH=M igration time (min)123(d)2 4 6 8 10864202468μeof (108m2V1s1)pH Bare capillary HEC Cat HEC 1 Cat HEC 2 Cat HEC 30 2 4 6 8 10 12 14 CatHEC2 coatingBare capillary M igration time (min)123HEC coatingElectroosmotic flow as a function of pH. Comparison between a bare fusedsilica capillary, HEC and catHEC coated capillary Electropherograms of a mixture of standard proteins. Separations were carried out using catHEC2 coated capillary 1 = Lysozyme。 2 = Cytochrome C。 3 = Ribonuclease A. Electropherograms of a mixture of standard proteins at pH . 0 2 4 6 8 10 12M igration time (min)PH=first runfiftieth run(a)1230 2 4 6 8 10 12 14M igration time ( min)first runfiftieth runpH=(b) 1230 2 4 6 8M igration time (min)first runfiftieth runpH=(c)123Protein Bare capillary N (plate/m) b HEC N (plate/m) RSD (%) CatHEC2 N(plate/m) RSD (%) Cytochrome C 9300 104000 186000 Lysozyme 162022 265000 Ribonuclease A 17300 180000 282022 Electrophoresis, 2022, 29, 14601466. Electroph
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