第九章抗生素的生產(chǎn)-資料下載頁
2025-01-06 13:38本頁面
【正文】 hear increased impellar diameter pensates for reduced agitation Axial Radial Impellars Different Impellar Designs 5. Heat Exchanger Heat generated by biological reactions agitation is removed by cooling jackets. Limpet, or half pipe, is mon choice Sparger Cshaped preferable to single pipe, as they improve O2 transfer in the viscous broths Cold H2O In Warm H2O Out 7. Analytical Instrumentation Probes for measurement of temp, pH glucose DO conc. are fitted at various points around the vessel ? Optimum temp between 24280C ? Alkaline pH must be reduced to neutrality ? Glucose must be monitored to prevent catabolite repression ? DO must sustain cells but promote fermentation 抗生素的提取 一般用溶媒體取法：利用抗生素在不同的 ph條件下的化學(xué)狀態(tài)（游離酸，堿或鹽）存在時(shí)，在水及與水互不相容的溶媒中溶解度不同的特性，使抗生素從一液相轉(zhuǎn)移到另一液相中（如有機(jī)溶劑），已達(dá)到濃酸和提純的目的。 Downstream Processing Steps in Downstream Processing: Downstream Processing ? Removal of Insolubles Filtration ? Isolation of product Solvent Extraction ?Purification Precipitation, Electrophoresis ? Polishing crystallisation, drying ? Penicillin G is in solution exocellularly Filtration ? Remove cells by filtration ? Sterile conditions must be utilised to avoid contamination of filtrate with Blactamase producing microanisms. ? Components dissolved in aqueous fermentation broth are recovered by extraction into an appropriate solvent: LiquidLiquid extraction Solvent Extraction ? Solvents: amyl acetate（醋酸戊酯） , butyl acetate（醋酸丁酯） and methyl isobutyl ketone（甲基異丁酮） at pH . ? Penicillin then extracted back into an aqueous buffer at pH Crystallisation and Lyophilisation ? An excess of potassium or sodium acetate is added in a mixing tank to induce crystallization. A centrifuge or drum filtration unit then collects the crystals which are washed and dried to produce the final product. ? Commercial scale lyophilisation (freezedrying) is used to dry and preserve the sensitive biological product. ? For parenteral use, the antibiotic is packed in sterile vials as a powder or suspension. ? For oral use, the antibiotic is tabletted with a film coating. Crystal Structur
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