【正文】 njection, no treatment). ? 跟據(jù)子宮內(nèi)膜炎的嚴重程度（輕微、嚴重）和治療方法（子宮內(nèi)灌注、前列腺注射、未治療）對病例進行分組。 The cases were grouped by and by the severity of endometritis (mild, severe) the treatment performed it did not differ 結(jié)果 與患有輕微子宮內(nèi)膜炎的奶牛相比，患有嚴重子宮內(nèi)膜炎的奶牛的妊娠率較低，而子宮內(nèi)灌注和前列腺注射治療患有嚴重子宮內(nèi)膜炎的奶牛的妊娠率之間無顯著性差異。 Results Conception rate was lower endometritis in cows with severe pared to cows with mild endometritis while between treatment groups. 輕微子宮內(nèi)膜炎奶牛經(jīng)子宮內(nèi)灌注治療后的空懷期顯著長于前列腺素注射組和對照組。 In cows with mild endometritis a significantly longer calvingtoconception interval was observed if intra uterine infusions were performed other two treatment options. pared to the conception rate and calvingtoconception intervals. ?相同配種期中所發(fā)生的其它生育問題對妊娠率和空懷期有顯著的影響。 The occurrence of other fertility problems during the same service period had a significant effect on ovarian or endocrine problem. ?結(jié)論 我們的結(jié)論是患有嚴重子宮內(nèi)膜炎的奶牛需要進行子宮內(nèi)灌注或前列腺素注射治療，而對于輕微病例，奶牛子宮自身康復(fù)潛力非常高，只要沒有另外的卵巢或內(nèi)分泌問題，在未經(jīng)治療的情況下，患病動物可以表現(xiàn)出好的繁殖性能。 Conclusion We conclude that with severe endometritis cows need to be treated with intrauterine infusions or prostaglandin whereas in mild cases the selfhealing potential is very high and affected animals show good reproductive efficiency without treatment as long as they do not have an additional ?關(guān)鍵詞： 子宮內(nèi)膜炎；子宮內(nèi)灌注；前列腺素注射；奶牛；繁殖性能 Keywords: endometritis, intrauterine infusions, prostaglandin injections, cow, reproductive efficiency 犢牛腹瀉的被動免疫：腸毒素性大腸桿菌 K99抗原和輪狀病毒的疫苗接種 Passive Immunity in Calf Diarrhea: Vaccination K99 Antigen of Enterotoxigenic Escherichia coli with and Rotavirus challenging newborn calves with ETEC. 摘要：目的 本研究旨在采用腸毒素性大腸桿菌 K99菌毛結(jié)合輪狀病毒疫苗免疫妊娠奶牛，并通過血清學(xué)分析和腸毒素性大腸桿菌對新生犢牛的攻毒試驗評估疫苗的免疫效果。 Abstract Objective The objective of this study was from enterotoxigenic Escherichia coli （ ETEC） K99 pili with a rotavirus vaccine for pregnant cows and to bine to assess the efficacy serology by and by and six cows were unvaccinated as controls. ?方法 使用腸毒素性大腸桿菌 K99提取物和失活輪狀病毒對 24頭妊娠奶牛進行肌肉注射免疫： 6頭奶牛分別注射有 2毫升油佐劑疫苗； 6頭奶牛分別注射有 ； 6頭奶牛分別注射有 4毫升氫氧化鋁佐劑疫苗， 4周后再注射一次； 6頭奶牛未注射疫苗作為對照組。 Methods from ETEC inactivated rotavirus intramuscularly with K99 extract and Twentyfour pregnant cows were vaccinated as follows: six cows were injected with 2 ml of oil adjuvanted vaccine。 six cows were injected with ml of oil adjuvanted vaccine。 six cows were injected with 4 ml of aluminum hydroxideadjuvanted vaccine twice with a fourweek interval。 until at least 28 days after calving. ? 24頭妊娠奶牛的新生犢牛于出生后 618小時進行腸毒素性大腸桿菌的攻毒試驗。 born to these cows Calves were challenged with ETEC at 6h to 18 h after birth. ? 結(jié)果 接種任一劑量油佐劑疫苗奶牛的血清和牛奶中的 K99和輪狀病毒抗體含量顯著增加，這一趨勢至少持續(xù)到產(chǎn)犢后的 28天。 Results to K99 and rotavirus in cows vaccinated with either dose of oil vaccine Serum and milk antibodies were significantly increased ? 接種氫氧化鋁佐劑疫苗的奶牛在血清和初乳而不是常乳中表現(xiàn)出 K99抗體的顯著增加，僅在初乳中表現(xiàn)出乳狀病毒抗體的顯著增加。 only in colostrum. In cows vaccinated with aluminum hydroxideadjuvanted vaccine, in serum and in colostrum but not in milk there was a significant K99 antibody increase and a significant rotavirus antibody increase ? 三組疫苗免疫妊娠奶牛分娩的 18頭犢牛中沒有一頭犢牛發(fā)生臨床型大腸桿菌病，腸毒素性大腸桿菌攻毒菌株隨糞便的排出量也有所減少。 ?未經(jīng)免疫接種妊娠奶牛分娩的六頭犢牛經(jīng)腸毒素性大腸桿菌的攻毒后有五頭犢牛發(fā)生腸毒素大腸桿菌病，六頭犢牛均排泄出腸毒素性大腸桿菌的攻毒菌株。 born to unvaccinated cows Five of six calves enterotoxic colibacillosis developed after challenge, and the challenge strain of ETEC. all excreted in the three vaccinated groups None of the 18 calves developed clinical colibacillosis, and anism was reduced. fecal excretion of the challenge ? Keywords： calf Diarrhea， K99 antigen， vaccination，enterotoxigenic Escherichia coli， rotavirus ? 結(jié)論 腸毒素性大腸桿菌 輪狀病毒復(fù)合疫苗有利于預(yù)防某些犢牛腹瀉的暴發(fā)。 Conclusion A bined ETECrotavirus vaccine may prove useful in preventing some outbreaks of calf diarrhea. ? 關(guān)鍵詞： 犢牛腹瀉； K99抗原；接種；腸毒素性大腸桿菌；輪狀病毒