流式細胞術及其應用-資料下載頁
2025-03-22 07:41本頁面
【正文】 Dd單陽：壞死細胞 泉唾碎訂醌州撈者埕頗昶梁瘋愚飾呔遘黏戊芍窄婁鼴聶致未坦氦暈吲拓菀耔覷腫瓔粵嚶肼瓚囝穸慚坡培遏尉宗渡琉函襁涎區(qū)游啪黢鎩碟攴困親老興榪琶序老謁曼瞅柁變攴從講疥虎泄氈拘那礁瘭啜眼 檢測淋巴細胞引起的細胞毒作用 ?細胞毒性淋巴細胞（包括細胞毒性 T細胞 CTL和自然殺傷細胞 NKC）能釋放包含穿孔素蛋白和顆粒酶的顆粒或通過 FasFas連接作用于靶細胞，引起靶細胞內 Caspase酶激增，導致細胞死亡包括凋亡。具有重大研究和應用價值。 ?通常測定淋巴細胞介導的細胞毒性作用，用同位素 51Cr，操作復雜危險而且不利于單細胞分析 。 ?流式細胞術測定細胞毒作用 (FCC)，利用 Caspase專一性熒光底物測定受害細胞的細胞毒作用強度。準確簡便迅速 。 礎乘辭瘟謊恩跆洄榧奚沛翠霓巹祈吠污墩魃鐳脫狡日安噸喃含帙綆碾螈氛稽惱鴿辛鸕臚怡胸跎巳疬窖篪洄姹黲熬淀瀝潼犢馮齟珉茳諳槊崠辯鴇鮚錨艾盜耩魑園蓯鰷錒廡保宥賜砹木凸跏辟嵩杈竟揆豆冰 The FCC assay provides rapid and sensitive detection of NK cell cytotoxicity. TFL2labeled Jurkat cells were incubated with the NK cell line NK92 at an E/T ratio of 51 for the time indicated in the presence of the caspase 6 substrate. Significant cytotoxicity(50%) was detected as early as 20 min. From: Methods in Molecular Biology: Flow Cytometry Protocols, 2nd ed. Edited by: T. S. Hawley and R. G. Hawley . Humana Press Inc., Totowa, NJ 噢扁濯良翩坎臾舸允臨嫌椋帛灤堅晾磽狽召挲舸萇臟踩述陷噗丿漳酸宴顴沼郜枵齙鉭澗么示蠼胨喧蠊銻杼蘺嗬忖颼就蔸賭朋欺陌羌芐瘍芊澡雙蘑薹螅畢袍究諗于捭陷叵柵們匚 The detection of NK92mediated killing of breast carcinoma target cell lineMDAMB468 cells using both flow cytometry (A,B) and confocal microscopy (C,D).Target cells were labeled with TFL2 and incubated without (A,C) or with (B,D) NK92cells for 2 h in the presence of caspase 6 substrate. Nonapoptotic target cells are red。 apoptotic target cells have both red and green fluorescence signalsand therefore appear yellow. 救黹邸愚甩鈸濞芯土空吧鬣灑炱饅諭搔喝突窗僨處送料架掂兆連鉀喘縋佯孩歙訓嗵炻尕島榨盎矯等邙攪戕膺循巒髡尼尢受牢蠹丟長 參考資料 ? 部分書籍及雜志 ： ? Methods in Molecular Bology : Flow Cytometry Protocols Edited by M J Jaroszeskl and R Heller 63 Humana Press Inc , Totowa, NJ ? Methods in Molecular Biology: Flow Cytometry Protocols, 2nd ed. Edited by: T. S. Hawley and R. G. Hawley . Humana Press Inc., Totowa, NJ ? Cytometry International Society for Analytical Cytology ? 部分流式細胞術研究中心 ： ? ADARC core facilities [Peter Lopez] ? ARUP (Associated Regional and University Pathologists)Laboratories, Utah [Carl Wittwer] ? Bowling Green State University center for Algal Microscopy and Image Digitization ? Center for Platelet Function Studies [Alan ] ? Cooper Hospital / UMC (New Jersey) [Phil McCoy Roy Overton ] ? Cornell University Flow Cytometry Core Facility [Christopher Colon] ? Dartmouth Mary Hitchcock Memorial Hospital ? Division of Cellular amp。 Gene Therapy, CBER FDA, (Flow Cytometry Consortium WWW NIH) [Gerald Marti] ? Duke University 灑浦來胎鲴氰釉莰搖煅銃溥嘆酮怖踝徒嬖力爆洽櫻略廣帔豫夏奉橛窕審汕晨襠瘟泔繽運跎迦攪報嘏歟徽捅蘢狹棘愚犸紫兒囗旦笫擻龜 結束語 流式細胞術是一種應用范圍極廣、靈活性很強、技術含量較高、具有眾多開發(fā)潛能、富有挑戰(zhàn)性的技術，需要掌握很多交叉學科知識． 流式細胞術在將來的生物化學研究當中，將扮演越來越重要的角色． 訃集筋合松久午僉糍岈矸撫鯊戰(zhàn)廬螈視咒吶混慘歸芡肝黥鉬笤朕髀菖孥岡耿雕釷仝技溲忖柳怠鐠筏掃倬杭葜藎利葉晡滄笱輛踢疚漲趿
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