疫學(xué)常用實(shí)驗(yàn)方法ppt課件-資料下載頁(yè)
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【正文】 長(zhǎng)分化，形成包含三個(gè)胚層來(lái)源的良性畸胎瘤 基因芯片： 分析基因表達(dá)差異 Culture of isolated stem cells 以鼠胚胎干細(xì)胞培養(yǎng)為例： 1，飼養(yǎng)細(xì)胞的培養(yǎng) 2，飼養(yǎng)層的制備 — MEF分離 3，小鼠胚胎干細(xì)胞分離培養(yǎng) 1）內(nèi)細(xì)胞團(tuán)的分離與培養(yǎng) 2） ES樣細(xì)胞擴(kuò)增 3）單細(xì)胞克隆建系 4）鑒定 — 絲裂霉素 C處理或 G γ射線照射 Differentiation of Stem Cells 懸浮培養(yǎng)法 — 不同的培養(yǎng)基及細(xì)胞因子 共培養(yǎng) — 與不同的支持細(xì)胞共培養(yǎng) III. Animal experiment ? Immune deficient mice 裸小鼠 (nude mice)，無(wú)毛，先天性胸腺發(fā)育不良， T細(xì)胞功能缺失。 SCID： Severe bined immune deficiency 嚴(yán)重聯(lián)合免疫缺陷小鼠 T、 B淋巴細(xì)胞功能均缺失 A variety of knockout mice: μMT, RAG1/, RAG2/ IFNγ/, IL4R/ … Transgenic mice: GFPtransgenic mice Generation of transgenic mice ?Animal experiments Isolate lymphocytes from spleen or lymph nodes Isolate tumor cells from tumor tissue. Adoptive transfer: T, B, sera… Bone Marrow Chimera ?Advanced animal experiment Skin graft Thank You! Topics ? Immunemediated angiostasis in tumor development. ? MDSC (myeloidderived suppressor cells) in tumor development. ? Th17 cells in tumor development. ? Treg cells in tumor development. ? Fibroblast in tumor development. ? The role of TNF and its receptors in tumor immunity. ? The role of Interferonγ and its receptor in tumor immunity. ? The role of Interleukin 4 and its receptor in tumor immunity. ? Cytokines and inflammation resolution. ? The role of liver fibrosis in development of hepatocellular carcinoma. ? 要求： ? 每組從 topic列表中任選一篇相關(guān)文章（ Journal of Immunology, Nature, Nature Immunology, The Journal of Clinical Investigation, Cancer Research, Science, The Journal of Experimental Medicine, Nature Medicine。 Blood, PNAS, Immunity, Cancer Cell.) ? Introduction部分要著重闡述， Results部分要說(shuō)清實(shí)驗(yàn)設(shè)計(jì)思路及過(guò)程結(jié)果， Discussion部分要指出創(chuàng)新點(diǎn)。 選 6名 speakers, 2名 chairmen ? 20分講， 10分討論
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