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. Describe the principle of Sanger’s enzymic method for DNA sequencing.3. What are the basic features of DNA double helix deduced by Watson and Crick?4. Please draw a schematic structure of TTP in the following space. You may write just the letter T for the base.5. On the following agarose gel with marker DNA as indicated:a) Place a plus (+) sign on the positive pole of the gel and a minus (–) sign on the negative pole.b) Draw the band or bands that you would see if you digested a 3 kb plasmid with EcoRI and ran it to the left of the marker. Assume the plasmid has a MCS with the following restriction sites: BamHI, EcoRI, EcoRV, HindIII, and KpnI.V Discuss the following topic (10 points)Describe your understanding of Central Dogma in detail. 第5頁(yè) 共5