醫(yī)學(xué)]mirnasandstemcell-資料下載頁
2025-01-08 01:16本頁面
【正文】 ion efficiencies by ~20 fold. Adding both cMyc and miR294 at the same time had no effect, suggesting that one reason behind the enhancing effect of cMyc on reprogramming is the induction of ESCspecific miRs miRNAs 和 ESC 的分化 Dicerdeficient ES cells fail to differentiate Size of teratomas following injection of the indicated ES cells into nude mice is plotted as a function of time (weeks postimplantation). Semiquantitative RT–PCR analyses of mesoderm and ectodermspecific differentiation markers. Tuj1+ cells (neurons) GFAP+ cells (astrocytes) miR9 miR9/ miR124 in neuronal system miR9 was inhibited alone (2′OMe9) or together with miR124a miRNAs（ miRNA1/miRAN133） 和心血管系統(tǒng)分化 原位雜交技術(shù)觀察發(fā)育過程中 miR1和果蠅肌細(xì)胞前體細(xì)胞（ Dmef2+）分化的關(guān)系 miRNA1通過抑制 Notch singnaling抑制心臟發(fā)育 miRNA overexpression 使 cardioblast 從 6個(gè)減少到 4個(gè) miRNA overexpression 使肌前體細(xì)胞（ Dmef+）數(shù)目減少 使果蠅翅膀靜脈數(shù)目增加，但四肢變短 miR134 levels alone in mouse ES cells enhanced differentiation toward ectodermal lineages, whereas inhibition of miR134 blocked differentiation miR134 and ectodermal differentiation miR134 Induces Morphological Changes microRNA (miR)134 modulates transcript levels of lineagespecific biomarkers, downregulates protein levels of pluripotency markers transfection of PmiR134 into E14 and D3 mESCs led to an appreciable upregulation of ectodermal markers Nestin and Fgf5 after 3 days, with a conitant reduction in endodermal, mesodermal, and pluripotencyassociated markers. 心血管系統(tǒng)的 miRNAsmiRNA1/miRAN133 miR1和 miR133具有同源性，同屬于 miR1家族 miR11和 miR133a2位于同一染色體上，且在胚胎發(fā)育過程中共表達(dá) miR12和 miR133a1位于同一染色體上，且在胚胎發(fā)育過程中共表達(dá) miR133b與 miR206同一染色體上。 miR206與內(nèi)皮細(xì)胞相關(guān) miRNA1/miRAN133的調(diào)節(jié) MyoD, myocyte enhancer factor2(Mef2), and serumresponse factor (SRF) MyoD：骨骼肌細(xì)胞 Mef2/SRF：骨骼肌細(xì)胞、心肌細(xì)胞、平滑肌細(xì)胞 miRNAs（ miRNA1/miRAN133） 和心血管系統(tǒng)發(fā)育 心臟早期發(fā)育 Dicer敲除導(dǎo)致 斑馬魚原腸期缺陷、腦形態(tài) /體節(jié)發(fā)育 /心臟發(fā)育缺陷而死亡 小鼠在胚胎期 （ display significant morphological abnormalities, reduced size, and greatly reduced expression of both Oct4, a hallmark of pluripotent stem cells, and Tbrachyury, a marker for differentiating mesodermal lineages of stem cells） +細(xì)胞特異性敲除 Dicer導(dǎo)致小鼠胚胎 成年動(dòng)物心臟 Dicer敲除導(dǎo)致猝死率顯著增加 miR12基因敲除導(dǎo)致 miR126和血管內(nèi)皮細(xì)胞 miRNAs和心臟疾病 miR195和心臟疾病 中度過表達(dá)（ alphaMHC為 promotor）使心臟變大，室壁變厚，室腔擴(kuò)大類似心室高負(fù)荷時(shí)的心機(jī)重構(gòu) 重度過表達(dá) miR195使心室擴(kuò)大，室壁變薄、 miR195為心臟負(fù)荷敏感性 miRNA（當(dāng)心臟負(fù)荷增加時(shí) miR195表達(dá)增加） miRNAs和心肌重構(gòu) miR208和心臟纖維化 心臟負(fù)荷增加時(shí)， miR208敲除 消除高負(fù)荷時(shí)心臟的纖維化
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