part-ii-23-重組(編輯修改稿)
2025-02-11 02:41 本頁面
【文章內(nèi)容簡介】 forming a singlestrand tail.3. RecA and SSB coat the tail.4. RecA promotes invasion of another DNA duplex, forming a Dloop. RecA helps the invading strand scan for a region of homology in the recipient DNA duplex.5. The invading strand basepaired with a homologous region, releasing SSB and RecA.6. RecBCD nicks the loopingout strand. RecA and SSB helps strand exchange.8. Branch migration occours, sponsored by RuvA and RuvB.9. RuvC resolves the structure.7. The nicks are sealed by DNA ligase, yielding a Holliday junction.四 . 真核生物的同源重組重組發(fā)生在第一個減數(shù)分裂的前期五 . 通過同源重組進(jìn)行基因敲除 位點特異性重組 Sitespecific Rebination發(fā)生在 DNA特異性位點上的重組。? Asthenameimplies,thistypeofrebinationinvolvestheexchangeofgeicmaterialatveryspecificsitesonly.? RequireslesssequencehomologybetweenrebiningDNAsthandoeshomologousrebination.? Specializedproteinsareneededtorecognizethesesitesandtocatalyzetherebinationreactionatthesesites.The steps and features * Strand exchange * Formation of a holliday intermediate * Branch migration * Resolution between two DNA molecularswithin one DNA molecularInverted repeatsDirect repeats The structure of rebinase recognitionsiteInverted repeatsDirect repeats . ? 噬菌體 DNA整合到 E. Coli DNA? att (attachment site) attB BOB’ ? phageattP POP’? Int (integrase)? IHF (integration host factor)~30bp~255bp? Xis (excisionase)Inverted repeatsSitespecific integration of lInverted repeatsDirect repeats Mechanism of Int action系列轉(zhuǎn)酯反應(yīng) 轉(zhuǎn)座重組 Transposition RebinationDNA上的一段核苷酸序列從一個位置轉(zhuǎn)移到另一個位置的現(xiàn)象。轉(zhuǎn)座子（ transposons)：發(fā)生轉(zhuǎn)位的 DNA片斷 Barbara McClintock spent many years studying the behavior of unusual geic elements in maize. She concluded that these elements were, in fact, mobile. Her work, all the more amazing because much of it was carried out before the structure of DNA was solved, was largely ignored until the mid 1970s when similar elements were discovered in bacteri
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