【文章內容簡介】 on) Morphology Consultations Blood and Marrow Cytogeics (in collaboration with the Cytogeics Laboratory) Molecular Diagnostics (in collaboration with the Molecular Diagnostics Laboratory) ? Lymphoma淋巴瘤 ? Flow Cytometry (Phenotypic Characterization, Staging, Residual Disease Detection, Fine Needle Aspirates) Morphology Consultations Lymph Node and other Tissue Biopsies Immunohistochemistry Molecular diagnostics (in collaboration with the Molecular Diagnostic Laboratory) ? Specialty Tests特殊檢查 ? CD34 Counting PNH (Paroxysmal Nocturnal Hemoglobinuria) Diagnosis陣發(fā)性睡眠性血紅蛋白尿 ? Bacteriology 細菌 ? Molecular Microbiology 分子微生物 ? Mycobacteriology 結核菌 ? Mycology 真菌 ? Parasitology 寄生蟲 ? Virology 病毒 ? Special Pathogen Detection – Actinomyces spp.: Culture。 PCR – Bordetella pertussis: Culture。 PCR – Legionella pneumophila: Culture。 Direct fluorescent antibody。 PCR – Mycoplasma pneumoniae: PCR – Mycoplasma hominis/Ureaplasma urealyticum: Culture – Bartonella spp.: Culture。 PCR – Chlamydia pneumoniae: PCR – Chlamydia trachomatis: Culture。 PCR – Ehrlichia/Anaplasma: PCR – Borrelia burgdorferi: Culture。 PCR – Neisseria gonorrheae: Culture。 PCR ? Bacterial Identification – Gasliquid chromatography of glucose metabolic end products for anaerobe identification. – Cell wall fatty acid analysis for speciation of nonfermenting or unmon isolates. – 16S ribosomal RNA gene sequence analysis for identification. ? Toxin Testing – Staphylococcus aureus PantonValentine leukocidin – Shigalike toxins 1 and 2 – Clostridium difficile toxin by antigen and cytotoxic methods ? Antibiotic Susceptibility Testing and Monitoring – Agar dilution susceptibility testing (MIC) – Antibiotic bacteriocidal determination (MIC/MBC) – Antibiotic serum activity (Schlichter test MID/MBD) – Antibiotic synergy testing – Antibiotic serum level determinations – Molecular determination of antibiotic resistance genes – Methicillin resistance (mecA) – Vanycin resistance (vanA。 vanB。 vanC) ? Molecular Epidemiology – Pulsedfield gel electrophoresis (PFGE) – Restriction fragmentlength polymorphisms (RFLP) ? EpsteinBarr virus (EBV) detection and quantification by PCR ? Parvovirus B19 PCR detection ? BK virus detection and quantification by PCR ? JC virus detection by PCR ? Cytomegalovirus (CMV) PCR detection ? VaricellaZoster virus (VZV) PCR detection ? Hepatitis C virus (HCV) quantification by PCR ? HCV genotype identification by direct sequencing ? Hepatitis B virus quantification by PCR ? Adenovirus detection by PCR ? Enterovirus detection by PCR ? High Risk HPV detection (HC2, Digene) ? Ehrlichia chaffeensis and Anaplasma phagocytophila (HGE agent) PCR detection ? Atypical pneumonia pathogen detection by PCR (Mycoplasma pneumoniae, Legionella pneumophila, Chlamydia pneumoniae) ? Bacterial identification by 16S ribosomal RNA gene sequencing ? Molecular epidemiology by PFGE or RFLP ? Staphylococcus enterotoxin and TSST1 gene and Shigalike toxin gene identification by PCR ? Bordetella pertussis PCR Detection ? Mycobacteriology ? Mycobacteria species identification and susceptibility testing, especially for patients with multiply resistant pathogens, is beyond the routine testing available in most hospital laboratories. The Johns Hopkins Hospital Mycobacteriology Laboratory offers the following testing: ? Culture/smear ? Speciation ? Mycobacterial blood culture ? Routine susceptibility testing for M. tuberculosis ? Susceptibility testing for select nonspecific mycobacteria upon request ? PCR for M. tuberculosis from smear positive respiratory (only) specimens ? Mycology ? Most clinical laboratories can only identify mon fungi, but the Hospital39。s diverse patient base gives the Microbiology Laboratory experience testing for speciation of unusual anisms and for performing yeast in vitro susceptibility testing. ? Fungal identification (yeast, dimorphics, filamentous) 真菌鑒定 ? Cryptococcus antigen detection (隱球菌抗原檢測） ? Fungal culture （真菌培養(yǎng)） ? Direct microscopic examination （直接鏡