全國(guó)大學(xué)生數(shù)學(xué)模型聯(lián)試題的解課程(編輯修改稿)
2025-02-10 01:06 本頁(yè)面
【文章內(nèi)容簡(jiǎn)介】 Parameter Standard Variable Estimate Error Type II SS F Value Pr F Intercept .0001 nn .0001 pp kk .0001 np .0001 nk .0001 Bounds on condition number: , 由此可得回歸方程若再加上市場(chǎng)價(jià)格就能尋求最佳施肥方案?練習(xí)題為了制造豬飼料,采用4種輔料,使用量分別是X1X4,相應(yīng)的豬飼料產(chǎn)量是y,試驗(yàn)16次,得到結(jié)果如下表?找出X1X4的最好二次多項(xiàng)式,用來(lái)預(yù)報(bào)Y?方案序號(hào)X1X2X3X4y110127531048510410724552012610620247572048458207251093012551030244101130487101230726513501241014502455155048651650727109AcknowledgmentsThe authors would like to thank Johns Hopkins University for the TC1 cells. This work was supportedby a National Health Research Institutes intramural grant (IV103PP22) and grants from the NationalScience Council, which were awarded to . Song (NSC 992321B400004MY3) and . Liu (NSC1032321B400008).Author Contributions. and . designed the studies. . performed the research and analyzed the data. . and. wrote the manuscript.Additional InformationC57BL/6 mice were immunized subcutaneously (.)once with 1 μ g of peptide mixed with or without 10 μ g of CpG adjuvant. After one week, splenocytes were
harvested, and the response of IFNγ secreting cells was determined by ELISPOT after 48 h of peptidestimulation. Briefly, 2 105 splenocytes were incubated with 1 μ g/ml irrelevant peptide or RAH peptidein an antiIFNγ coated polyvinylidene fluoride (PVDF) plate for 48 h. After incubation, the cells wereremoved, and a biotinylated antiIFNγ Ab (eBioscience, San Diego, CA, USA) was added to each well.The plates were incubated at 37 176。C for 2 h. Following the addition of the avidinHRP reagent (eBioscience,CA, USA), the assay was developed using a 3amine9ethyl carbazole (AEC。 SigmaAldrich, MO,USA) staining solution. The reaction was stopped after 4–6 min by placing the plate under tap water.The spots were counted using an ELISPOT reader (Cellular Technology Ltd., Shaker Heights, OH, USA).For RAHspecific T cell staining, spleens were harvested seven days after the immunizations, andRAHspecific CD8+ T cells were detected by tetramer staining using a PElabeled RAH tetramer(Beckman Coulter, CA, USA) and a FITClabeled antiCD8 monoclonal antibody (mAb) (eBioscience,CA, USA). The stained RAHspecific CD8+ T cells were analyzed by flow cytometr
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