dns-氨基酸的雙向聚酰胺薄膜層析(編輯修改稿)
2025-06-15 12:51 本頁面
【文章內(nèi)容簡介】 事項(xiàng) （ 1）嚴(yán)格控制點(diǎn)樣位置以及點(diǎn)樣直徑。 （ 2）展層時(shí)勿將點(diǎn)樣浸入溶劑系統(tǒng)。 （ 3）展層后必須經(jīng)電吹風(fēng)將膜吹干。 （ 4）使用紫外照射時(shí)要注意使用時(shí)間短 . 注意 Procedure for TLC 1. Prepare the developing container. The developing container for TLC can be a specially designed chamber, a jar with a lid, or a beaker with a watch glass on the top: Pour solvent into the beaker to a depth of just less than cm. To aid in the saturation of the TLC chamber with solvent vapors, line part of the inside of the beaker with filter paper Cover the beaker with a watch glass, swirl it gently, and allow it to stand while you prepare your TLC plate. TLC plates used in the anic chem teaching labs are purchased as 5 cm x 20 cm sheets. Each large sheet is cut horizontally into plates which are 5 cm tall by various widths。 the more samples you plan to run on a plate, the wider it needs to be. Prepare the TLC plate. Shown in the photo to the left is a box of TLC plates, a large uncut TLC sheet, and a small TLC plate which has been cut to a convenient size. Plates will usually be cut and ready for you when you e to lab. Handle the plates carefully so that you do not disturb the coating of adsorbent or get them dirty. Measure cm from the bottom of the plate. Take care not to press so hard with the pencil that you disturb t
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