微生物實驗論文-wenkub
2022-11-13 06:40:03 本頁面
【正文】 oyu, Zong Lu School of Life Science, Shandong University, Jinan,250100 Corresponding author Abstract In our experiment, we took an appropriate amount of soil sample, dissolved them in distilled water, put them in enrichment culture, and then sifted the bacteria and fungus which can break down cellulose by Congo redcellulose medium. After that, we did the pure cultures to observe the color, shape and some other characteristics of the colonies, moreover, we stained the bacteria we got with Gram stain. Later we did some physiological and biochemical experiments such as methyl red test, amyl hydrolysis experiment and pH gradient test to see whether these bacteria can produce acid, whether they can hydrolyze starch and their ability of breaking down cellulose under different PH valves. We hope that by doing this experiment, we can lay the foundation for further research in the future. Keywords Soil, Cellulosedeposing microbes, Isolation and screening, PH experiment 前言在工業(yè)化水平日益提高、科學技術飛速發(fā)展的今天，我們同時也面臨著世界人口激增、礦產(chǎn)資源枯竭等嚴峻的問題。因此，開發(fā)高效轉化木質(zhì)纖維素類可再生資源的微生物技術，將生產(chǎn)生活中產(chǎn) 生的工農(nóng)業(yè)廢棄物轉化為可利用的新能源，將具有極其重要的意義和廣闊的前景。本次試驗就是的目的就是從土壤中將這些能夠分解纖維素的細菌分離出來，并對它們的形態(tài)特征、生理生化等進行研究和探討。另外，土樣的采集要選擇富含纖維素的環(huán)境，這是因為在纖維素含量豐富的環(huán)境，通常會聚集較多的分解纖維素的微生物。 富集培養(yǎng)所需要的儀器有： 250ml 錐形瓶、天平、藥匙、玻璃棒、電爐、搖床、玻璃珠、稱量紙等。在250ml 錐形瓶中裝入 50ml 培養(yǎng)基，放 5— 6顆玻璃珠，用 8 層紗布做成瓶塞，將瓶口塞緊，再在瓶塞外包裹兩層牛皮紙，用線繩扎緊，在 121℃下高壓蒸汽滅菌 20min。 用量程為 1000181。 需要滅菌的儀器：無菌培養(yǎng)皿（ 12 個）、涂布器（ 3 個）、移液器、槍頭。劃線完畢后，蓋上 皿蓋，倒置于溫室培養(yǎng)，至長出單菌落。經(jīng)此方法染色后，細胞保留初染劑藍紫色的細菌為革蘭氏陽性菌；如果細胞中初染劑被脫色劑洗脫而使細菌染上復染劑的顏色 (紅色 )，該菌屬于革蘭氏陰性菌。所以染色時的條件要嚴格控制。 所需儀器或試劑：菌種 1， 2 的平板培養(yǎng)
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