【正文】 無水溶性產(chǎn)生 無水溶性產(chǎn)生金氏等 B 培養(yǎng)基 無非水溶性色素產(chǎn)生 無非水溶性色素產(chǎn)生 病原菌的個體形態(tài)特征通過普通光學(xué)顯微鏡對菌株 SS2 的菌體形態(tài)進(jìn)行觀察，結(jié)果（見表 、圖、圖 、圖 ）表 菌株 SS2 個體形態(tài)的比較菌株染色方法S1 S2革蘭氏染色 革蘭氏陰性，短桿狀 革蘭氏陰性，短桿狀，近似球狀CTE 722BCTE 722C食用菌工廠化生產(chǎn)污染菌的研究12芽孢染色 無芽孢 無芽孢圖 菌株 S1 的芽孢染色結(jié)果 16S rDNA 序列分析為進(jìn)一步確定菌株的分類位置，對其 16S rDNA 序列進(jìn)行了分析，結(jié)果見表下表 菌株 S1 的 16S rDNA 序列分析表 菌株 S1 的 16S rDNA 序列ACGCTGGCGG CAGGCCTAAC ACATGCAAGT CGAGCGGATG AGAAGAGCTT 50GCTCTTCGAT TCAGCGGCGG ACGGGTGAGT AATGCCTAGG AATCTGCCTG 100GTAGTGGGGG ACAACGTTTC GAAAGGAACG CTAATACCGC ATACGTCCTA 150CGGGAGAAAG CAGGGGACCT TCGGGCCTTG CGCTATCAGA TGAGCCTAGG 200TCGGATTAGC TAGTTGGTGA GGTAATGGCT CACCAAGGCG ACGATCCGTA 250ACTGGTCTGA GAGGATGATC AGTCACACTG GAACTGAGAC ACGGTCCAGA 300CTCCTACGGG AGGCAGCAGT GGGGAATATT GGACAATGGG CGAAAGCCTG 350ATCCAGCCAT GCCGCGTGTG TGAAGAAGGT CTTCGGATTG TAAAGCACTT 400TAAGTTGGGA GGAAGGGCA TAACCTAATA CGTTAGTGTT TTGACGTTAC 450食用菌工廠化生產(chǎn)污染菌的研究13TCGACAGAATA AGCACCGGCT AACTCTGTGC CAGCAGCCGC GGTAATACAG 500AGGGTGCAAG CGTTAATCGG AATTACTGGG CGTAAAGCGC GCGTAGGTGG 550TTTGTTAAGT TGGATGTGAA AGCCCCGGGC TCAACCTGGG AACTGCATCC 600AAAACTGGCA AGCTAGAGTA CGGTAGAGGG TGGTGGAATT TCCTGTGTAG 650CGGTGAAATG CGTAGATATA GGAAGGAACA CCAGTGGCGA AGGCGACCAC 700CTGGACTGAT ACTGACACTG AGGTGCGAAA GCGTGGGGAG CAAACAGGAT 750TAGATACCCT GGTAGTCCAC GCCGTAAACG ATGTCAACTA GCCGTTGGAA 800TCCTTGAGAT TTTAGTGGCG CAGCTAACGC ATTAAGTTGA CCGCCTGGGG 850AGTACGGCCG CAAGGTTAAA ACTCAAATGA ATTGACGGGG GCCCGCACAA 900GCGGTGGAGC ATGTGGTTTA ATTCGAAGCA ACGCGAAGAA CCTTACCAGG 950CCTTGACATG CAGAGAACTT TCCAGAGATG GATTGGTGCC TTCGGGAACT 1000CTGACACAGG TGCTGCATGG CTGTCGTCAG CTCGTGTCGT GAGATGTTGG 1050GTTAAGTCCC GTAACGAGCG CAACCCTTGT CCTTAGTTAC CAGCACGTTA 1100TGGTGGGCAC TCTAAGGAGA CTGCCGGTGA CAAACCGGAG GAAGGTGGGG 1150ATGACGTCAA GTCATCATGG CCCTTACGGC CTGGGCTACA CACGTGCTAC 1200AATGGTCGGT ACAGAGGGTT GCCAAGCCGC GAGGTGGAGC TAATCTCACA 1250AAACCGATCG TAGTCCGGAT CGCAGTCTGC AACTCGACTG CGTGAAGTCG 1300GAATCGCTAG TAATCGCGAA TCAGAATGTC GCGGTGAATA CGTTCCCGGG 1350CCTTGTACAC ACCGCCCGTC ACACCATGGG AGTGGGTTGCA CCAGAAGTAG 1400CTAGTCTAAC CTTCGGGAGG ACGGTTACCA CGGTGTGATT CATGACTGGG 1450結(jié)果：根據(jù)以上各個結(jié)果，得出：S1 菌株為假單胞菌屬 菌株 S2 的 16S rDNA 序列分析表 菌株 S1 的鑒定結(jié)果Accession Description Max scoreTotal scoreQuery coverageE valueMax ident Uncultured bacterium clone N167 16S ribosomal RNA gene, partial sequence Pseudomonas sp. BSw10041N 16S ribosomal RNA, partial sequence2680 2680 100% 100%食用菌工廠化生產(chǎn)污染菌的研究14 gb|| Pseudomonas sp. PG12 16S ribosomal RNA gene, partial sequence2680 2680 100% 100% Pseudomonas sp. WMQ7 16S ribosomal RNA gene, partial sequence2680 2680 100% 100% Uncultured bacterium clone MP1040916b40 16S ribosomal RNA gene, partial sequence2680 2680 100% 100% Uncultured Pseudomonas sp. clone SQ9_Pitesti 16S ribosomal RNA gene, partial sequence2680 2680 100% 100% Pseudomonas putida gene for 16S rRNA, partial sequence, strain: KF7152680 2680 100% 100% Pseudomonas putida gene for 16S rRNA, partial sequence, strain: NBRC 141642676 2676 100% 99% Pseudomonas sp. OCR2 gene for 16S rRNA, partial sequence2676 2676 100% 99% Pseudomonas putida strain ATCC 12633 16S ribosomal RNA gene, partial sequence2676 2676 100% 99% Uncultured bacterium clone N07 16S ribosomal RNA gene, partial sequence 2675 2675 100% 99% Uncultured Pseudomonas sp. clone AV_5NG03 16S ribosomal RNA gene, partial sequence2675 2675 100% 99% Pseudomonas sp. WSCIII 16S ribosomal RNA gene, partial sequence2675 2675 100% 99% Pseudomonas putida strain ATCC 11172 16S ribosomal RNA gene, partial sequence2675 2675 100% 99% Uncultured bacterium clone 1H3C_22 16S ribosomal RNA gene, partial sequence 2669 2669 100% 99% Pseudomonas sp. CSNA6 16S ribosomal RNA gene, partial sequence2669 2669 100% 99% Uncultured bacterium clone N163 16S ribosomal RNA gene, partial sequence 2669 2669 100% 99%表 菌株 S2 的 16S rDNA 序列ACGCTGGCGG CAGGCCTAAC ACATGCAAGT CGAGCGGTAG AGAGAAGCTT 50GCTTCTCTTG AGAGCGGCGG ACGGGTGAGT AATGCCTAGG AATCTGCCTG 100GTAGTGGGGG ATAACGTTCG GAAACGGACG CTAATACCGC ATACGTCCTA 150CGGGAGAAAG CAGGGGACCT TCGGGCCTTG CGCTATCAGA TGAGCCTAGG 200TCGGATTAGC TAGTTGGTGG GGTAATGGCT CACCAAGGCG ACGATCCGTA 250ACTGGTCTGA GAGGATGATC AGTCACACTG GAACTGAGAC ACGGTCCAGA 300CTCCTACGGG AGGCAGCAGT GGGGAATATT GGACAATGGG CGAAAGCCTG 350ATCCAGCCAT GCCGCGTGTG TGAAGAAGGT CTTCGGATTG TAAAGCACTT 400食用菌工廠化生產(chǎn)污染菌的研究15TAAGTTGGGA GGAAGGGTTG TAGATTAATA CTCTGCAATT TTGACGTTAC 450CGACAGAATA AGCACCGGCT AACTCTGTGC CAGCAGCCGC GGTAATACAG 500AGGGTGCAAG CGTTAATCGG AATTACTGGG CGTAAAGCGC GCGTAGGTGG 550TTTGTTAAGT TGGATGTGAA ATCCCCGGGC TCAACCTGGG AACTGCATTC 600AAAACTGACT GACTAGAGTA TGGTAGAGGG TGGTGGAATT TCCTGTGTAG 650CGGTGAAATG CGTAGATATA GGAAGGAACA CCAGTGGCGA AGGCGACCAC 700CTGGACTAAT ACTGACACTG AGGTGCGAAA GCGTGGGGAG CAAACAGGAT 750TAGATACCCT GGTAGTCCAC GCCGTAAACG ATGTCAACTA GCCGTTGGAA 800GCCTTGAGCT TTTAGTGGCG CAGCTAACGC ATTAAGTTGA CCGCCTGGGG 850AGTACGGCCG CAAGGTTAAA ACTCAAATGA ATTGACGGGG GCCCGCACAA 900GCGGTGGAGC ATGTGGTTTA ATTCGA