【正文】
核心酶 Pol III Pol III’ Pol III* γ 復 合 體 β sliding clamp 二、解螺旋酶 (helicase): A helicase is an enzyme that uses energy provided by NTP hydrolysis to separate the strands of a nucleic acid duplex 三、 SSB蛋白(單鏈結合蛋白) The singlestrand binding protein (SSB) attaches to singlestranded DNA, thereby preventing the DNA from forming a duplex ?防止單鏈復性 ?維持單鏈剛性狀態(tài) ?避免單鏈降解 ?The SSB binds as a monomer, but typically in a cooperative manner in which the binding of one protein molecule makes it much easier for another to bind 四、引發(fā)酶 (primase): The primase is a type of RNA polymerase that synthesizes short segments of RNA that will be used as primers for DNA replication. 為什么 DNA的合成需要 RNA引物? 五、旋轉酶 (gyrase ): a